Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
REACTIVE AQUEOUS TWO-PHASE PARTITION OF CELLULASE
Korean Journal of Chemical Engineering, October 1993, 10(4), 235-239(5), 10.1007/BF02705273
Download PDF
Abstract
Reactive aqueous two-phase partition of cellulase by modification with amphiphilic copolymer was studied. The modifier of cellulase could be directly used as a polymer of two-phase system. The maleic acid anhydride functional group of the synthetic copolymer covalently coupled with the amino groups of cellulase molecule. The degree of modification of the amino groups was controlled by the types of synthetic copolymer of the weight ratio of a synthetic copolymer to cellulase. As the degree of modification of cellulase increased, the modified cellulase moved to the copolymer phase of the copolymer/dextran two-phase system.
References
Habeeb AFSF, Anal. Biochem., 14, 328 (1966)
Hartree MM, Hogan CM, Saddler JN, Biotechnol. Bioeng., 30, 558 (1987)
Kajiuchi T, Park JW, J. Chem. Eng. Jpn., 25, 202 (1992)
Kajiuchi T, Park JW, Proc. of APBIoCHEC '92, 106 (1992)
Mandels M, Andreotti R, Roche C, Biotechnol. Bioeng., 6, 21 (1976)
Miller GL, Anal. Chem., 31, 426 (1959)
Kajiuchi T, Park JW, Moon HY, J. Chem. Eng. Jpn., 26, 28 (1993)
Park JW, Lee SM, Kajiuchi T, J. Chem. Eng. Jpn., 23, 497 (1990)
Moloney A, Coughlan MP, Biotechnol. Bioeng., 25, 271 (1983)
Ooshina H, Sakata M, Harano Y, Biotechnol. Bioeng., 28, 1727 (1986)
Fields R, Biochem. J., 124, 581 (1971)
Beldman G, VanLeeuwen MFS, Rombauts FM, Voragen FGJ, Eur. J. Biochem., 146, 302 (1985)
Shikata S, Nishizawa K, Biochem. J., 78, 499 (1978)
Tanaka M, Appl. Microbiol. Biotechnol., 23, 263 (1986)
Hartree MM, Hogan CM, Saddler JN, Biotechnol. Bioeng., 30, 558 (1987)
Kajiuchi T, Park JW, J. Chem. Eng. Jpn., 25, 202 (1992)
Kajiuchi T, Park JW, Proc. of APBIoCHEC '92, 106 (1992)
Mandels M, Andreotti R, Roche C, Biotechnol. Bioeng., 6, 21 (1976)
Miller GL, Anal. Chem., 31, 426 (1959)
Kajiuchi T, Park JW, Moon HY, J. Chem. Eng. Jpn., 26, 28 (1993)
Park JW, Lee SM, Kajiuchi T, J. Chem. Eng. Jpn., 23, 497 (1990)
Moloney A, Coughlan MP, Biotechnol. Bioeng., 25, 271 (1983)
Ooshina H, Sakata M, Harano Y, Biotechnol. Bioeng., 28, 1727 (1986)
Fields R, Biochem. J., 124, 581 (1971)
Beldman G, VanLeeuwen MFS, Rombauts FM, Voragen FGJ, Eur. J. Biochem., 146, 302 (1985)
Shikata S, Nishizawa K, Biochem. J., 78, 499 (1978)
Tanaka M, Appl. Microbiol. Biotechnol., 23, 263 (1986)
