Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Mathematical modeling for the estimation of lipase activity by agar diffusion method

Chang WJ Moon YH Koo YM

Korean Journal of Chemical Engineering, May 1997, 14(3), 179-183(5), 10.1007/BF02706092

Download PDF

Abstract

A mathematical model was developed to estimate the lipase activities from the experimentally obtained physical dimension of halo, formed by a diffusing lipase through agar media. A diffusion equation base upon the mass balance was derived in cylindrical coordinates, the integration of which was carried out by using finite difference method. A regressional analysis of the experimental data using the proposed model has brought the values for the hindered diffusivity of lipase through agar media and the minimum effective lipase concentration of 0.817×10^-6 cm²/s and 2.86 unit/cm³, respectively. The procedures, delineated in this work are considered to be useful for the quantitative analysis of haloforming enzymes at predetermined standard conditions.

Keywords

Lipase Activity Halo Agar Modeling

References

Constantinides A, "Applied Numerical Methods with Personal Computers," McGraw-Hill, New York (1987)
Fransmana M, Junne G, Roobeek A, "The Biotechnology Revolution?" Blackwell, Oxford (1995)
IMSL, "IMSL-LIBRARY User's Manual, Vol. 4," IMSL, Houston (1984)
Kouker G, Jaeger KE, Appl. Environ. Microbiol., 53(1), 211 (1987)
Lee SY, Rhee JS, J. Microbiol. Biotechnol., 4(2), 85 (1994)