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COMPARISON AND OPTIMIZATION OF POLY(3-HYDROXYBUTYRATE) RECOVERY FROM Alcaligenes eutrophus AND RECOMBINANT Escherichia coli

Hahn SK Ryu HW Change YK

Korean Journal of Chemical Engineering, January 1998, 15(1), 51-55(5), 10.1007/BF02705305

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Abstract

The recovery of poly(3-hydroxybutyrate) [PHB] from Alcaligenes eutrophus and a recombinant Escherichia coli strain harboring the A. eutrophus poly(3-hydroxyalkanoates) biosynthesis genes was studied. When PHB was recovered using sodium hypochlorite or sodium dodecyl sulfate (SDS), non-PHB cell materials (NPCM) of the recombinant E. coli seemed to be more easily digested than those of A. eutrophus. Furthermore, viscosity increase caused by cell lysis during SDS treatment was negligible for the recombinant E. coli, whereas a very viscous suspension was formed for A. eutrophus. These results, together with our previous finding that PHB in the recombinant E. coli is far less susceptible to molecular degradation by sodium hypochlorite, suggested that the recombinant E. coli was more beneficial than A. eutrophus in teams of PHB recovery. In order to develop an easy and efficient recovery process, we adopted and optimized the SDS treatment since, with the hypochlorite treatment, we could not handle high biomass concentrations effectively. We could obtain a PHB of 95 % purity with 96 % recovery under the optimal condition of a biomass concentration of 5 %, a ratio of SDS to biomass of 0.6, a treatment time of 60 minutes, and a treatment temperature of 30℃.

Keywords

Poly(3-hydroxybutyrate Alcaligenes eutrophus Recombinant Escherichia coli Sodium Hypochlorite Sodium Dodecyl Sulfate

References

Barham PG, Keller A, Otum EL, Holmes PA, J. Mater. Sci., 19, 2781 (1984)
Berger E, Ramsay BA, Ramsay JA, Chavarie C, Braunegg G, Biotechnol. Tech., 3, 227 (1989)
Braunegg G, Sonnleitner B, Lafferty RM, Eur. J. Appl. Microbiol. Biotechnol., 6, 29 (1978)
Byrom D, Trends Biotechnol., 5, 246 (1987)
Hahn SK, Chang YK, Kim BS, Lee KM, Chang HN, Biotechnol. Tech., 7, 209 (1993)
Hahn SK, Chang YK, Lee SY, Appl. Environ. Microbiol., 61, 34 (1995)
Holmes PA, Phys. Technol., 16, 32 (1985)
Holmes PA, Lim GB, "Separation Process," U.S. Patent, 4,910,145 (1990)
Kim BS, Cho GD, Oh JT, Kim WS, HWAHAK KONGHAK, 32(3), 441 (1994)
Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI, Biotechnol. Bioeng., 43(9), 892 (1994)
Koo YM, Parekh BS, Hatfield GW, Lim HC, Korean J. Chem. Eng., 13(2), 202 (1996)
Lafferty RM, Heinzle E, "Use of Cyclic Carbonic Acid Esters as Solvents for Poly(β-hydroxybutyric Acid)," U.S. Patent, 868,795 (1979)
Lee SY, Chang HN, Chang YK, Ann. NY Acad. Sci., 721, 43 (1994)
Lee SY, Lee KM, Chang HN, Steinbuchel A, Biotechnol. Bioeng., 44(11), 1337 (1994)
Lee SY, Yim KS, Chang HN, Chang YK, J. Biotechnol., 32, 203 (1994)
Mayer JM, Kaplan DL, Trends Biotechnol., 2, 227 (1994)
Middelberg AP, Lee SY, Martin J, Williams DR, Chang HN, Biotechnol. Lett., 17(2), 205 (1995)
Page WJ, Cornish A, Appl. Environ. Microbiol., 59, 4236 (1993)
Poirier Y, Nawrath C, Somerville C, Biotechnol., 13, 142 (1995)
Ramsay JA, Berger E, Ramsay BA, Chavarie C, Biotechnol. Tech., 4, 221 (1990)
Swift G, Accounts Chem. Res., 26, 105 (1993)
Yim KS, Lee SY, Chang HN, Korean J. Chem. Eng., 12(2), 264 (1995)