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A TISSUE EMBEDDING TECHNIQUE FOR MEASURING THE STRUCTURE OF HAIRY ROOT MATS OF Tagetes erecta
Korean Journal of Chemical Engineering, March 1998, 15(2), 150-156(7), 10.1007/BF02707067
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Abstract
A tissue embedding protocol which preserves root diameter was developed for hairy root mats of Tagetes erecta. Embedded root mats were sectioned, stained and the cut surfaces were subjected to image analysis to determine the void fraction of the root mats. Void fractions of mats of different ages, grown from inocula containing different numbers of tips, were analyzed. The method was found to give reproducible values for the void fractions, exhibiting reasonable trends with respect to initial tip number and mat age.
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Ashford AE, Allaway WG, Grubler F, Lennon A, McCully ME, J. Microsc., 144, 107 (1986)
Ashford AE, McCully ME, Protoplasma, 70, 441 (1970)
Coetzee J, VanDer Merwe CF, J. Microsc., 137, 129 (1985)
Cole MB, J. Microsc., 127, 139 (1982)
Glauert AM, "Fixation, Dehydration and Embedding of Biological Specimens," Practical Methods in Electron Microscopy, Glauert, A.M. (eds.), Vol. 3, North-Holland (1974)
Kamada H, Okamura N, Sataka M, Harada M, Shimomara K, Plant Cell Reports, 5, 239 (1986)
Lawton JR, Harris PJ, J. Microsc., 112, 307 (1978)
Leduc E, Holt SJ, J. Cell Biol., 26, 137 (1965)
Luft JH, Wood RL, J. Cell Biol., 19, 46 (1963)
Mukundan U, Hjortso MA, J. Exp. Bot., 41, 1497 (1990)
O'Brien TP, Kuo J, McCully ME, Zee SY, Aust. J. Biol. Sci., 26, 1231 (1973)
Prince CL, Bringi V, Shuler ML, Biotechnol. Prog., 7, 195 (1991)
Sabatini DD, Miller F, Barrnett RJ, Appl. Cytochem. Electron Microsc., 8, 57 (1963)
Scala J, Schwab D, Simmons E, Am. J. Bot., 55, 649 (1968)
Sharp JM, Doran PM, J. Biotechnol., 16, 171 (1990)
Toivonen L, Biotechnol. Prog., 9, 12 (1993)
Whitney PJ, Enzyme Microb. Technol., 14, 13 (1992)
Yu SX, Doran PM, Biotechnol. Bioeng., 44(8), 880 (1994)
