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Pulse Response Test in Medium Pressure Protein Chromatography
Korean Journal of Chemical Engineering, November 1998, 15(6), 575-579(5), 10.1007/BF02698980
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Abstract
An MPLC (Medium Pressure Liquid Chromatography) column was in-house packed with an anion exchange silica gel (PAE 1000, Amicon) in order to carry out pulse response experiments to investigate MPLC column efficiency. Bovine serum albumin was isocratically eluted from the column with varying ionic strength of NaCl, and the chromatograms obtained were analyzed by the moment method. Broadening of the elution band was caused mostly by a longitudinal dispersion in our system, and no significant numerical differences in HETP with the velocity change were observed at the high ionic strength.
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