Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Gradient Separation of Soybean Phospholipids with Retention Factors of a New Polynomial Correlation
Korean Journal of Chemical Engineering, March 1999, 16(2), 170-174(5), 10.1007/BF02706832
Download PDF
Abstract
The separation mechanism of solutes under gradient conditions has been studied. The separation of a mixture of soybean phospholipids (phosphatidylethanolamine, phosphatidyl-inositol, and phosphatidylcholine) in the ternary mobile phase of methanol, hexane, and isopropanol was investigated. An analytical expression was obtained to calculate the retention volume under a two step-gradient mode. The following new polynomial equation was also presented, log k''=A+B×F+C×F2+D×G+E×G2 where A, B, C, D, and E are empirical coeffieients and F and G are volume percentage of isopropanol and methanol in the mobile phase based on hexane, respectively. The resulting equations of the three phospholipids were used to describe the effect of the composition of the ternary mobile phase on the retention factor under step-gradient conditions.
References
Abidi SL, Mounts TL, J. Liquid Chromatogr., 17, 105 (1994)
Abidi SL, Mounts TL, Rennick KA, J. Liquid Chromatogr., 17, 3705 (1994)
D'Agostino G, O'Hare MJ, Mitchell F, Salomon T, Verillon F, Chromatographia, 25, 343 (1988)
Hathout Y, Maume G, Maume BF, J. Chromatogr. Biomed. Appl., 652, 1 (1994)
Hurst WJ, Martin RA, Sheeley RM, J. Liquid Chromatogr., 9, 2969 (1986)
Kim JD, Row KH, So MS, Polunina IA, Larin AV, J. Liquid Chromatogr., 18, 3091 (1995)
Klein BH, Dudenhausen JW, J. Liquid Chromatogr., 17, 981 (1994)
Markowski W, Golkiewicz W, Chromatographia, 25, 339 (1988)
Row KH, Lee JW, Korean J. Chem. Eng., 14(5), 412 (1997)
Snyder LR, Quarry MA, J. Liquid Chromatogr., 10, 1789 (1987)
van der Meeren P, van der Deelen J, Huys M, Baert L, J. Am. Oil Chem. Soc., 67, 815 (1990)
Xie M, Zhou C, Ren Z, Luo T, J. Chromatogr., 555, 33 (1991)
Abidi SL, Mounts TL, Rennick KA, J. Liquid Chromatogr., 17, 3705 (1994)
D'Agostino G, O'Hare MJ, Mitchell F, Salomon T, Verillon F, Chromatographia, 25, 343 (1988)
Hathout Y, Maume G, Maume BF, J. Chromatogr. Biomed. Appl., 652, 1 (1994)
Hurst WJ, Martin RA, Sheeley RM, J. Liquid Chromatogr., 9, 2969 (1986)
Kim JD, Row KH, So MS, Polunina IA, Larin AV, J. Liquid Chromatogr., 18, 3091 (1995)
Klein BH, Dudenhausen JW, J. Liquid Chromatogr., 17, 981 (1994)
Markowski W, Golkiewicz W, Chromatographia, 25, 339 (1988)
Row KH, Lee JW, Korean J. Chem. Eng., 14(5), 412 (1997)
Snyder LR, Quarry MA, J. Liquid Chromatogr., 10, 1789 (1987)
van der Meeren P, van der Deelen J, Huys M, Baert L, J. Am. Oil Chem. Soc., 67, 815 (1990)
Xie M, Zhou C, Ren Z, Luo T, J. Chromatogr., 555, 33 (1991)
