Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Adsorptive Behavior of a Globular Protein with a Monoglyceride Monolayer Spread on the Aqueous Surface

Daechul Cho^† Michel Cornec

Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-1146, U.S.A., USA

dcho@lgen.co.kr

Korean Journal of Chemical Engineering, May 1999, 16(3), 371-376(6), 10.1007/BF02707127

Download PDF

Abstract

The dynamics of surface pressure (π) and of surface concentration (Γ) of a ¹⁴C radiolabeled β-lactoglobulin, a globular protein, adsorbed onto a spread monolayer of 1-monopalmitoyl-rac glycerol (named commonly as a monoglyceride), at the air-water interface were measured. The adsorption of ¹⁴C labeled β lactoglobulin was enhanced at short times when C-16 monoglyceride of 73.4 and 24.7 μg was spread on the aqueous surface. However, the amounts of protein adsorbed at the steady state (after 10 h) were lowered with the values being 0.8 and 1.2 mg/m², respectively. Spreading of such amount of monoglyceride that forms a dense packed monolayer onto air-water interface led to complete displacement of the protein from the aqueous surface after 2.5 h, possibly because of the surface pressure and exclusion effects.

Keywords

Lipid-Protein Interaction Monolayer Monoglyceride β-Lactoglobulin

References

Bos MA, Nylander T, Langmuir, 12(11), 2791 (1996)
Boury F, Ivanova T, Panaiotov I, Proust JE, Langmuir, 11(2), 599 (1995)
Boury F, Ivanova T, Panaiotov I, Proust JE, Langmuir, 11(6), 2131 (1995)
Brewkink E, Demel RA, De Korte-Kool G, De Kruijff B, Biochemistry, 31, 1119 (1992)
Cho D, Narsimhan G, Franses EI, Langmuir, 13(17), 4710 (1997)
Clark DC, Mackie AR, Wilde PJ, Wilson DR, Faraday Discuss, 98, 253 (1994)
Clark DC, Wilde PJ, Wilson DR, Colloids Surf., 59, 209 (1991)
Coke M, Wilde PJ, Russel EJ, Clark DC, J. Colloid Interface Sci., 138, 489 (1990)
Cornec M, Mackie AR, Wilde PJ, Clark DC, Colloids Surf. A: Physicochem. Eng. Asp., 114, 237 (1996)
Cornell DG, Carroll RJ, J. Colloid Interface Sci., 108, 226 (1985)
Cornell DG, Patterson DL, J. Agric. Food Chem., 37, 1455 (1989)
Cornell DG, J. Colloid Interface Sci., 88, 536 (1982)
Cornell DG, Patterson DL, Hoban N, J. Colloid Interface Sci., 140, 428 (1990)
Courthaudon JL, Dickinson E, Matsumara Y, Clark DC, Colloids Surf., 56, 293 (1991)
Du Y, An J, Tang J, Jiang L, Colloids Surf. B: Biointerfaces, 7, 129 (1996)
Ewers WE, Sutherland KL, Aust. J. Sci. Res. Ser. A, 5, 697 (1952)
Gehlert U, Weidemann G, Vollhardt D, J. Colloid Interface Sci., 174(2), 392 (1995)
Grimard R, Tancrede P, Gicquaud C, Biochem. Biophys. Res. Com., 190, 1017 (1993)
Hunter JR, Kilpatrick PK, Carbonell RG, J. Colloid Interface Sci., 142, 429 (1991)
Ibdah JA, Phillips MC, Biochemistry, 27, 7155 (1988)
Israelachvili J, Colloids Surf. A: Physicochem. Eng. Asp., 91, 1 (1994)
Kozorac Z, Dhathathregan A, Mobius D, FEBS, 229, 372 (1988)
MacRitchie F, Adv. Protein Chem., 32, 283 (1978)
McClements DJ, "Food Emulsions: Principles, Practice and Techniques," CRC Press (1998)
Narsimhan G, Uraizee F, Biotechnol. Prog., 8, 187 (1992)
Nylander T, "Proteins at Liquid Interfaces," Mobius, D. and Miller, R. Eds., Elsevier (1998)
Quinn PJ, Dawson RMC, Biochem. J., 113, 791 (1969)
Rahman A, Sherman P, Colloid Polym. Sci., 260, 1035 (1982)
Nino MRR, Patino JMR, JAOCS, 75, 1233 (1998)
Nino MRR, Wilde PJ, Clark DC, Patino JMR, Langmuir, 14(8), 2160 (1998)
Sarker DK, Wilde PJ, Clark DC, Colloids Surf. B: Biointerfaces, 3, 349 (1995)
Wilde PJ, Clark DC, J. Colloid Interface Sci., 155, 48 (1993)
Xu S, Damodaran S, J. Colloid Interface Sci., 157, 485 (1993)