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Received December 18, 2000
Accepted April 4, 2001
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Separation of Perillyl Alcohol from the Peel of Citrus Unshiu by Supercritical CO2 and Preparative High-Performance Liquid Chromatography
Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University, Incheon 402-751, Korea 1Environment Remediation Research Center, KIST, Seoul 136-791, Korea
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, May 2001, 18(3), 352-356(5), 10.1007/BF02699177
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Abstract
To separate perillyl alcohol (POH), a potential anti-cancer agent, the peel of citrus unshiu was extracted by supercritical CO2 extraction (SCE) system at 50 ℃, 200 bar and 6 kg CO2/hr/kg sample. The extracts were partitioned by acetonitrile/hexane (90/10, vol%). POH was eluted in the acetonitrile phase. An open-tubular chromatography with silica gel (40-63 μm) was used to purify POH from the acetonitrile phase. Mobile phase was hexane/ethyl acetate (90/10, vol%). To obtain POH in a pure form, finally preparative high-performance liquid chromatography was applied. The collection of POH from citrus unshiu peel was achieved on a laboratory-prepared chromatographic column (300×3.9 mm) packed with 15 μm C18 preparative packings. The composition of mobile phase was water/acetonitrile (50/50, vol%). The flow rate of the mobile phase was 1 ml/min and UV wavelength was fixed at 205 nm. It was found that the total yield of POH was 1.6×10(-3) (wt%) as the dry powder of citrus unshiu peel.
References
Brandani V, Re GD, Giacomo GD, Muccinante V, Fluid Phase Equilib., 59, 135 (1990)
Reverchon E, Marciano A, Poletto M, Ind. Eng. Chem. Res., 36(11), 4940 (1997)
Gould MN, J. Cell. Biochem., 22, 139 (1995)
Jung YA, Row KH, Korean J. Chem. Eng., 15(5), 538 (1998)
Jung YA, Row KH, J. Chromatogr. A, 828, 445 (1998)
Kelloff GJ, Boone CW, Crowell JA, Steele VE, Lubet RA, Doody LA, Malone WF, Hawk ET, Sigman CC, J. Cell. Biochem., 63, 1 (1996)
Lee CH, Jung JY, Lee YW, Kim JD, Lee YY, Row KH, "Separation of Perillyl Alcohol in Korean Orange Peel by SFE and RP-HPLC," 24th International Symposium on High Performance Liquid Phase Separations and Related Techniques, P-1132, Seattle, Washington, USA, June 24-30 (2000)
Lee CH, Row KH, Lee YW, Kim JD, Lee YY, J. Liq. Chrom. Rel. Technol, in press (2001)
Lee YW, Lee CH, Kim JD, Lee YY, Row KH, Sep. Sci. Technol., 35(7), 1069 (2000)
Lee YW, Lee CH, Kim JD, Lee YY, Row KH, "Purification of Perillyl alcohol in Korean Orange Peel by supercritical Fluid Extraction," The 8(th) APCChE'99 Congress, 511 (1999)
Sato M, Goto M, Hirose T, Ind. Eng. Chem. Res., 34(11), 3941 (1995)
Row KH, Larin AV, J. Chem. Eng. Jpn., 28(6), 851 (1995)
Row KH, Larin AV, Korean J. Chem. Eng., 12(4), 442 (1995)
Temelli F, Chen CS, Braddock RJ, Food Technol., 42, 145 (1988)
Reverchon E, Marciano A, Poletto M, Ind. Eng. Chem. Res., 36(11), 4940 (1997)
Gould MN, J. Cell. Biochem., 22, 139 (1995)
Jung YA, Row KH, Korean J. Chem. Eng., 15(5), 538 (1998)
Jung YA, Row KH, J. Chromatogr. A, 828, 445 (1998)
Kelloff GJ, Boone CW, Crowell JA, Steele VE, Lubet RA, Doody LA, Malone WF, Hawk ET, Sigman CC, J. Cell. Biochem., 63, 1 (1996)
Lee CH, Jung JY, Lee YW, Kim JD, Lee YY, Row KH, "Separation of Perillyl Alcohol in Korean Orange Peel by SFE and RP-HPLC," 24th International Symposium on High Performance Liquid Phase Separations and Related Techniques, P-1132, Seattle, Washington, USA, June 24-30 (2000)
Lee CH, Row KH, Lee YW, Kim JD, Lee YY, J. Liq. Chrom. Rel. Technol, in press (2001)
Lee YW, Lee CH, Kim JD, Lee YY, Row KH, Sep. Sci. Technol., 35(7), 1069 (2000)
Lee YW, Lee CH, Kim JD, Lee YY, Row KH, "Purification of Perillyl alcohol in Korean Orange Peel by supercritical Fluid Extraction," The 8(th) APCChE'99 Congress, 511 (1999)
Sato M, Goto M, Hirose T, Ind. Eng. Chem. Res., 34(11), 3941 (1995)
Row KH, Larin AV, J. Chem. Eng. Jpn., 28(6), 851 (1995)
Row KH, Larin AV, Korean J. Chem. Eng., 12(4), 442 (1995)
Temelli F, Chen CS, Braddock RJ, Food Technol., 42, 145 (1988)
