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Received December 28, 2000
Accepted March 20, 2001
- This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Comparison of the Adsorption Characteristics of Expanded Bed Adsorbent with Conventional Chromatographic Adsorbent
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, 310027, China 1Department of Biological Engineering, Inha University, Inchon 402-751, China
Korean Journal of Chemical Engineering, May 2001, 18(3), 357-362(6), 10.1007/BF02699178
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Abstract
The static and kinetic adsorption characteristics of Streamline DEAE and DEAE-Sepharose FF were studied under various operating conditions. The adsorption isotherms for the two types of adsorbents were obtained and found to fit well to a Langmuir-type expression. The adsorption kinetics of Streamline DEAE at different concentrations, temperatures, and viscosities were studied and a mathematical model including particle size distribution was developed to describe the adsorption performance of Streamline DEAE. Comparing the uptake curves of Streamline DEAE with DEAE-Sepharose FF, it could be concluded that Streamline DEAE achieves equilibrium faster to get equilibrium than DEAE-Sepharose FF, indicating that Streamline DEAE could be used in higher flow rate systems.
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References
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Chase HA, Dreager MN, J. Chromatogr., 597, 129 (1992)
Chase HA, Trends Biotechnol., 12, 296 (1994)
Cho D, Cornec M, Korean J. Chem. Eng., 16(3), 371 (1999)
Choi JW, Korean J. Chem. Eng., 7(4), 269 (1990)
Clemmitt RH, Chase HA, Biotechnol. Bioeng., 67(2), 206 (2000)
Finlayson BA, "Nonlinear Analysis in Chemical Engineering," McGraw-Hill Inc., New York (1980)
Graham EE, Fook CH, AIChE J., 28, 245 (1982)
Hjorth R, Trends Biotechnol., 15, 230 (1997)
Horstmann BJ, Chase HA, Chem. Eng. Res. Des., 67, 243 (1989)
Hu HB, Yao SJ, Zhu ZQ, Chem. Eng.-Chinese, 27, 37 (1999)
Johnston A, Hearn MTW, J. Chromatogr., 512, 101 (1990)
Johnston A, Mao QM, Hearn MTW, J. Chromatogr., 548, 127 (1991)
Karau A, Benken C, Thommes J, Kula MR, Biotechnol. Bioeng., 55(1), 54 (1997)
Mullick A, Flickinger MC, Biotechnol. Bioeng., 65(3), 282 (1999)
Owen RO, Chase HA, Chem. Eng. Sci., 54(17), 3765 (1999)
Park IS, Kwak C, Hwang YG, Korean J. Chem. Eng., 17(6), 704 (2000)
Skidmore GL, Horstmann BJ, Chase HA, J. Chromatogr., 498, 113 (1990)
Tsou HS, Graham EE, AIChE J., 31, 1959 (1985)
Wright PR, Muzzio FJ, Glasser BJ, Biotechnol. Prog., 15(5), 932 (1999)