A method of isolation of bioactive water-soluble saponin-firee subfraction (RG-ws-1-1-1) from Korean red ginseng using solvent extraction, ultrafiltration and chromatography on Diaion HP-20 resin was developed. To evaluate the potency of RG-ws-1-1-1 to effect on the heat stress (HS) response and anti-inflammatory response in neutrophils, the oxygen consumption (OC) and formation of superoxide radicals (FSR) were assayed in suspensions of neutrophils from mice. Supplementation of RG-ws-1-1-1 to mice in vivo (200 mg/kg, 14 days intraperitoneally) results in the increase OC by neutrophils assayed ex vivo at 37 ℃. OC and FSR by neutropirils were suppressed when cells obtained from the control animals were incubated at 42 ℃. Preincubation of neutrophils with RG-ws-1-1-1 reduces HS related depression of FSR. Supplementation of RG-ws-1-1-1 to mice in vivo decreases HS related suppression of OC. RG-ws-1-1-1 subfraction significantly improves an oxygen-dependent anti-microbial function of neutrophils, also studied under the HS conditions.

Chromatographic Method Ultrafiltration Ginseng Neutrophils Heat Stress

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