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Received August 29, 2001
Accepted December 3, 2001
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Protein Separation by High-Performance Membrane Chromatography
Center for Advanced Bioseparation Techology and Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Inchon 402-751, Korea
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, March 2002, 19(2), 314-317(4), 10.1007/BF02698421
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Abstract
High-performance membrane chromatography (HPMC) is a very effective chromatographic method that combines the advantages of both membrane technology and column chromatography. In this work, proteins (myoglobin, conalbumin and soybean trypsin inhibitor) were separated by HPMC. The separation mechanism involved anionexchange, and the stationary phases were used anion CIM DEAE and QA disks (12×3 mm). Two types of mobile phase, buffer A (20 mM Tris-HCl, pH 7.4) and buffer B (buffer A+1 M NaCl) were used. As the amount of sodium chloride dissolved in buffer linearly increased, the retention time shortened and the resolution of the components was greatly improved. The optimum mobile phases and operating conditions were experimentally determined. From the experimental results, the proteins were separated within 2 min at a mobile phase flow rate of 4 ml/min.
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References
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Wensheng X, Fred ER, J. Chromatogr. A, 828, 357 (1998)
Zeng XF, Ruckenstein E, J. Membr. Sci., 148(2), 195 (1998)
Ales P, Milos B, Janez J, Ales S, J. Chromatogr. A, 848, 51 (1998)
Belenkii BG, Maltsev VG, Biotechniques, 18, 288 (1995)
Charcosset C, J. Chem. Technol. Biotechnol., 71(2), 95 (1998)
Dubinina NI, Kurenbin OI, Tennikova TB, J. Chromatogr. A, 753, 217 (1996)
Elisabeth H, J. Chromatogr. A, 852, 351 (1999)
Galina A, Tishchenko MB, Jici S, Tomas B, J. Chromatogr. B, 706, 157 (1998)
Joseph A, Gerstner RH, Cramer SM, J. Chromatogr., 596, 173 (1992)
Roper DK, Lightfoot EN, J. Chromatogr. A, 702, 3 (1995)
Laure G, Berreux RF, Tatiana BT, J. Pharm. Biomed. Anal., 24, 95 (2000)
Lee YW, So MS, Lee JW, Chung ST, Row KH, Korean J. Chem. Eng., 13(6), 578 (1996)
Lian S, Anderson DJ, J. Chromatogr. A, 909, 191 (2001)
Michael B, Tennikov N, Gazdina V, Tatiana B, Tennikova FS, J. Chromatogr. A, 798, 55 (1998)
Peter R, Levison AK, Hopkins PH, J. Chromatogr. A, 865, 3 (1999)
Peter R, Levison SE, Badger PH, Martin J, Davies IJ, Bruce VG, J. Chromatogr. A, 827, 337 (1998)
Shuichi Y, Eiji M, J. Chromatogr. A, 852, 25 (1999)
Shuichi Y, Peter K, Watler DF, Oliver K, J. Chromatogr. A, 852, 37 (1999)
Sridhar P, Chem. Eng. Technol., 19(5), 398 (1996)
Vodopivec M, Berovic M, Jancar J, Podgornik A, Strancar A, Anal. Chim. Acta, 407, 105 (2000)
Wensheng X, Fred ER, J. Chromatogr. A, 828, 357 (1998)
Zeng XF, Ruckenstein E, J. Membr. Sci., 148(2), 195 (1998)
