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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received November 22, 2001
Accepted May 15, 2002

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of Microbial Adaptation at Enzyme Level for Enhancing Biodegradation Rate of BTX

Sung-Ho Yeom^{1 2} Young-Je Yoo^1†

¹School of Chemical Engineering, Seoul National University, Seoul 151-742, Korea ²EnzyBiotech Inc., Technology Incubation Network, Seoul National University, Seoul 151-742, Korea

Korean Journal of Chemical Engineering, September 2002, 19(5), 780-782(3), 10.1007/BF02706967

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Abstract

Catechol was found to be a common intermediate in the degradation of benzene and toluene by Alcaligenes xylosoxidans Y234, and the ring cleavage of the catechol mediated by catechol 1,2-dioxygenase was a rate-determining step. Since benzene induced higher level of catechol 1,2-dioxygenase than toluene, the cells pre-adapted to benzene showed higher degradation rate of benzene and toluene. The degradation rate of m-xylene was also increased significantly when benzene-adapted cells were inoculated. m-Xylene was metabolized via 3-methyl catechol which was effectively cleaved by catechol 1,2-dioxygenase.

Keywords

BTX Microbial Adaptation Enzyme Induction Catechol 1,2-Dioxygenase Biodegradation

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