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Received January 22, 2002
Accepted March 10, 2002
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Separation of Phospholipids from Soybean by NP-HPLC with ELSD
Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, September 2002, 19(5), 818-820(3), 10.1007/BF02706974
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Abstract
Phospholipids of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) from lecithin were separated on gradient steps by NP-HPLC with ELSD. The ternary mobile phases of hexane, IPA, and water were used, and the optimum mobile phase conditions were experimentally determined. The calibration curves of phospholipids were constructed to analyze quantitatively the amounts of PE and PC. The responses of PE by ELSD showed linearity with the injected amount range but a logarithmic relationship of PC was observed. By quantitative analysis, 14.2% of PE and 36.6% (w/w) of PC were contained in the sample of lecithin. Finally, in the separation of phospholipids, the detection by ELSD was better than that by UV.
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References
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Servillo L, Iorio EL, Quagliuolo L, Camussi G, Balestrieri C, Giovane A, J. Chromatogr. B, 689, 281 (1997)
