Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
pH EFFECT ON THE SEPARATION OF -CHYMOTRYPSIN BY AFFINITY CHROMATOGRAPHY
Korean Journal of Chemical Engineering, March 1985, 2(1), 11-15(5), 10.1007/BF02697544
Download PDF
Abstract
For the separation of enzyme ( -chymotripsin) the characteristics of affinity chromatography were studied. The system employed Sepharose CL-4B as a gel matrix (a packing meterial), 4-phenylbutylamine as a ligand and -chymotrypsin as a solute.
Moment analysis was used to determine the retention time in a column, in which the longitudinal dispersion in the mobile phase, the radial diffusion inside the porous spherical gel beads and the sorption on the ligands attached to the gel bead were simultaneously taking place.
To analyze the effect of pH on the retention time an ionization model was incorporated into the chromatrgraphic model. Dependence of adsorption equilibrium constant and the retention time on pH were investigated. From the experimental data the approximate pK values could be obtained.
Moment analysis was used to determine the retention time in a column, in which the longitudinal dispersion in the mobile phase, the radial diffusion inside the porous spherical gel beads and the sorption on the ligands attached to the gel bead were simultaneously taking place.
To analyze the effect of pH on the retention time an ionization model was incorporated into the chromatrgraphic model. Dependence of adsorption equilibrium constant and the retention time on pH were investigated. From the experimental data the approximate pK values could be obtained.