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Received April 12, 2002
Accepted August 14, 2002
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Adsorption Performance of Proteins to CM Sepharose FF and DEAE Sepharose FF Adsorbents
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, 310027, China
Korean Journal of Chemical Engineering, January 2003, 20(1), 93-98(6), 10.1007/BF02697191
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Abstract
Adsorption equilibrium and kinetics were studied for the binding of proteins to CM Sepharose FF and DEAE Sepharose FF. The influence of temperature, pH, viscosity, initial concentration and the volume of adsorbents on the adsorption characteristics was investigated in detail. The results showed that the isotherms of lysozyme to CM Sepharose FF were well described by a Langmuir-type correlation. The two phase resistance model describing the dynamic adsorption process of lysozyme, papain, BSA to CM Sepharose FF was presented, and the pore diffusion_x000D_
coefficients were determined by using this model and the dynamic adsorption data.
References
Bonnerjea J, Oh S, Hoare M, Dunnill P, Bio/Technol., 4, 954 (1986)
Chase HA, J. Chromatogr., 297, 179 (1984)
Graham EE, Fook CH, AIChE J., 28(2), 245 (1982)
Hong SB, Row KH, Korean J. Chem. Eng., 19(2), 314 (2002)
Hu HB, Yao SJ, Zhu ZQ, Chem. Eng.(China), 29(1), 37 (2001)
Hu HB, Yao SJ, Zhu ZQ, Hur BK, Korean J. Chem. Eng., 18(3), 357 (2001)
Janson JC, Korean J. Chem. Eng., 18(2), 149 (2001)
Johnston A, Hearn MTW, J. Chromatogr., 512, 101 (1990)
Kim DW, Jang YH, Jeong YK, Biotechnol. Appl. Biochem., 27, 97 (1998)
Peterson EA, Sobers HA, J. Am. Chem. Soc., 78, 751 (1956)
Skidmore GL, Horstmann BJ, Chase HA, J. Chromatogr., 498, 113 (1990)
Tsou HS, Graham EE, AIChE J., 31(12), 1959 (1985)
Chase HA, J. Chromatogr., 297, 179 (1984)
Graham EE, Fook CH, AIChE J., 28(2), 245 (1982)
Hong SB, Row KH, Korean J. Chem. Eng., 19(2), 314 (2002)
Hu HB, Yao SJ, Zhu ZQ, Chem. Eng.(China), 29(1), 37 (2001)
Hu HB, Yao SJ, Zhu ZQ, Hur BK, Korean J. Chem. Eng., 18(3), 357 (2001)
Janson JC, Korean J. Chem. Eng., 18(2), 149 (2001)
Johnston A, Hearn MTW, J. Chromatogr., 512, 101 (1990)
Kim DW, Jang YH, Jeong YK, Biotechnol. Appl. Biochem., 27, 97 (1998)
Peterson EA, Sobers HA, J. Am. Chem. Soc., 78, 751 (1956)
Skidmore GL, Horstmann BJ, Chase HA, J. Chromatogr., 498, 113 (1990)
Tsou HS, Graham EE, AIChE J., 31(12), 1959 (1985)
