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In relation to this article, we declare that there is no conflict of interest.
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Received April 12, 2002
Accepted August 14, 2002
articles This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Adsorption Performance of Proteins to CM Sepharose FF and DEAE Sepharose FF Adsorbents

Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, 310027, China
Korean Journal of Chemical Engineering, January 2003, 20(1), 93-98(6), 10.1007/BF02697191
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Abstract

Adsorption equilibrium and kinetics were studied for the binding of proteins to CM Sepharose FF and DEAE Sepharose FF. The influence of temperature, pH, viscosity, initial concentration and the volume of adsorbents on the adsorption characteristics was investigated in detail. The results showed that the isotherms of lysozyme to CM Sepharose FF were well described by a Langmuir-type correlation. The two phase resistance model describing the dynamic adsorption process of lysozyme, papain, BSA to CM Sepharose FF was presented, and the pore diffusion_x000D_ coefficients were determined by using this model and the dynamic adsorption data.

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