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Received August 14, 2002
Accepted October 30, 2002
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Separation of Whey Proteins by Anion-Exchange Membranes
Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, May 2003, 20(3), 538-541(4), 10.1007/BF02705562
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Abstract
Three strong anion-exchange membranes (CIM QA, Q100 and HiTrap Q) were investigated for the_x000D_
separation of the major proteins, which were contained in whey, such as α-Lactalbumin, BSA and β-Lactoglobulin. Experiments were performed to determine the optimum mobile phase composition for separating the whey proteins using the standard chemicals of the proteins. The mobile phase was buffer A (20 mM piperazine-HCl pH 6.4) and buffer B (buffer A+1 M NaCl) and the linear gradient elution changes of salt concentration were applied. The standard chemicals of the proteins were used to investigate the optimal mobile phase compositions with the three anion-exchange membranes. From the experimental results, it was found that HiTrap Q was the most effective in separating whey proteins.
References
Andrew LZ, Int. Dairy J., 8, 243 (1998)
Choi DY, Row KH, HWAHAK KONGHAK, 40(4), 461 (2002)
Lucas D, Rabiller-Baudry M, Millesime L, Chaufer B, Daufin G, J. Membr. Sci., 148(1), 1 (1998)
Gerberding SJ, Byers CH, J. Chromatogr. A, 808, 141 (1998)
Gerd K, Barbel L, Wolfgang F, Int. Dairy J., 10, 713 (2000)
Hahn R, Schulz PM, Schaupp C, Jungbauer A, J. Chromatogr. A, 795, 277 (1998)
Hong SB, Row KH, Korean J. Chem. Eng., 19(2), 314 (2002)
Hutchens TW, Magnuson JS, Yip TT, J. Immunological Methods, 128, 89 (1990)
Jose GL, Alves F, Lucy DA, Luiza HM,daSilvaTelma TF, Antonio JAM, J. Chromatogr. B, 743, 235 (2000)
Keith DR, Edwin NL, J. Chromatogr. A, 702, 3 (1995)
Korhonen H, Pihlanto-Leppala A, Rantamaki P, Tupasela T, Trends Food Sci. Technol., 9, 307 (1998)
Lieske B, Konrad G, Int. Dairy J., 6, 13 (1996)
Lourdes A, Isidra R, Mercedes R, Int. Dairy J., 10, 135 (2000)
Luiza HM, Antonio JAM, Carbohydrate Polym., 42, 279 (2000)
Rito-Palomares M, Miguel H, J. Chromatogr. B, 711, 81 (1998)
ROW KH, Korean J. Chem. Eng., 6(3), 179 (1989)
Strange ED, Malin EL, VanHekken DL, Basch JJ, J. Chromatogr. A, 624, 81 (1992)
Ye X, Yoshida S, Ng TB, Int. J. Biochem. Cell Biology, 32, 1143 (2000)
Choi DY, Row KH, HWAHAK KONGHAK, 40(4), 461 (2002)
Lucas D, Rabiller-Baudry M, Millesime L, Chaufer B, Daufin G, J. Membr. Sci., 148(1), 1 (1998)
Gerberding SJ, Byers CH, J. Chromatogr. A, 808, 141 (1998)
Gerd K, Barbel L, Wolfgang F, Int. Dairy J., 10, 713 (2000)
Hahn R, Schulz PM, Schaupp C, Jungbauer A, J. Chromatogr. A, 795, 277 (1998)
Hong SB, Row KH, Korean J. Chem. Eng., 19(2), 314 (2002)
Hutchens TW, Magnuson JS, Yip TT, J. Immunological Methods, 128, 89 (1990)
Jose GL, Alves F, Lucy DA, Luiza HM,daSilvaTelma TF, Antonio JAM, J. Chromatogr. B, 743, 235 (2000)
Keith DR, Edwin NL, J. Chromatogr. A, 702, 3 (1995)
Korhonen H, Pihlanto-Leppala A, Rantamaki P, Tupasela T, Trends Food Sci. Technol., 9, 307 (1998)
Lieske B, Konrad G, Int. Dairy J., 6, 13 (1996)
Lourdes A, Isidra R, Mercedes R, Int. Dairy J., 10, 135 (2000)
Luiza HM, Antonio JAM, Carbohydrate Polym., 42, 279 (2000)
Rito-Palomares M, Miguel H, J. Chromatogr. B, 711, 81 (1998)
ROW KH, Korean J. Chem. Eng., 6(3), 179 (1989)
Strange ED, Malin EL, VanHekken DL, Basch JJ, J. Chromatogr. A, 624, 81 (1992)
Ye X, Yoshida S, Ng TB, Int. J. Biochem. Cell Biology, 32, 1143 (2000)
