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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received September 11, 2002
Accepted November 15, 2002

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kinetic Model for the Simulation of Hen Egg White Lysozyme Adsorption at Solid/Water Interface

Woo-Kul Lee^{1 2} Jea Seung Ko^1†

¹Laboratory for the Study of Molecular Biointerfaces, Intellectual Biointerface Engineering Center andDepartment of Oral Anatomy, College of Dentistry, BK21HLS, Seoul National University, 28-22, YeonKun-Dong, ChongRo-Ku, Seoul 110-749, Korea ²Department of Chemical Engineering, College of Engineering, Dankook University, Hannam-Dong, Yongsan-Ku, Seoul 140-714, Korea

jeasko@snu.ac.kr

Korean Journal of Chemical Engineering, May 2003, 20(3), 549-553(5), 10.1007/BF02705564

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Abstract

A simulation model for adsorption kinetics of hen egg white lysozyme (HEW) adsorption to hydrophilic silica is proposed. The adsorption kinetic data were monitored by using in-situ ellipsometry. The model is based on an irreversible adsorption mechanism allowing two different adsorbed states. The adsorbed states were differentiated based on binding strengths resistant to the concentration gradient exerted by rinse. Molecules desorbing and remaining upon rinse were identified as loosely bound (state 1) and tightly bound (state 2) states, respectively. The adsorption rate constants were assumed to be a time-dependent nonlinear function in order to account for the change in surface properties originating from the protein layer formed on the surface. The parameters of adsorption rate constants were evaluated by using adsorption kinetic data at different protein concentrations, and the relationships between the adsorption parameters and protein concentration were established which eventually demonstrated a linear relationship._x000D_ The established relations between the adsorption parameters and concentration elucidated the effect of protein concentration on adsorption to hydrophilic silica.

Keywords

Protein Adsorption Adsorbed State Lysozyme Ellipsometry Kinetic Model

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