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Received February 3, 2003
Accepted July 14, 2003
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Caffeine Molecular Imprinted Microgel Spheres by Precipitation Polymerization
1Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, Korea 2Department of Chemistry, Hebei University, Baoding 071002, Korea 3Department of Chemistry, Hebei University, Baoding 071002, China
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, November 2003, 20(6), 1073-1076(4), 10.1007/BF02706938
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Abstract
Imprinted uniform microgel spheres were prepared by precipitation polymerization. Acetonitrile was used as the dilute solvent with MAA as the monomer, EDMA as the crosslinker and caffeine as the print molecule. Comparison of caffeine adsorption on molecular imprinted and blank microgel spheres was made. Langmuir model was used to fit the adsorption data. It was found that the caffeine imprinted microgel spheres show specific binding sites to the target molecules. A binding study of caffeine on imprinted microgel spheres was made by Scatchard analysis; the dissociation constants (KD) and the maximum binding capacity were KD=1.84×10-4 mol/L, Qmax=16.98 μmol/g for high affinity binding site and KD=1.33×10-3 mol/L, Qmax=46.84 μmol/g for lower affinity binding site, respectively. This microgel spheres can be useful affinity adsorbents in further applications.
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Zhou J, He X, Li Y, Anal. Chim. Acta, 394, 353 (1999)
