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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received September 9, 2003
Accepted December 3, 2003

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Application of Image Analysis for Precise, Noninvasive Measurement of Plant Culture Growth

Eddie James In Sang Yoo James M. Lee^†

Department of Chemical Engineering, Washington State University, Pullman, Washington 99163, Korea

jmlee@wsu.edu

Korean Journal of Chemical Engineering, January 2004, 21(1), 195-200(6), 10.1007/BF02705398

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Abstract

Accurately measuring the growth of solid cultures is important for evaluating regrowth following low temperature storage, cell screening on selective media, or the suitability of different media formulations. However, current procedures are inadequate to provide precise, quantitative data non-invasively. This paper describes the development of an image analysis technique to provide quantitative growth data for callus cultures. Various properties obtained from image analysis, such as area, perimeter, diameter, and integrated density value, were correlated with the observed mass of plant callus. Based on this data, a predictive linear model was developed. The image analysis method was used to estimate cell mass as a function of various media components and growth conditions. This work demonstrates that image analysis is a flexible and useful tool for monitoring cultures.

Keywords

Image Analysis Plant Cells Callus Culture

References

Chi CM, Zhang C, Staba EJ, Cooke TJ, Hu WS, Biotechnol. Bioeng., 50(1), 65 (1996)
Kino-oka M, Prenosil JE, Biotechnol. Bioeng., 67(2), 234 (2000)
Magnuson NS, Linzmaier PM, Gao JW, Reeves R, An G, Lee JM, Protein Expr. Purif., 7, 220 (1996)
Mills DR, Lee JM, Plant Cell Reports, 15, 634 (1996)
Miyanaga K, Seki M, Furusaki S, Biotechnol. Bioeng., 67(4), 493 (2000)
Murashige T, Skoog F, Plant. Physiol., 15, 473 (1962)