Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received April 14, 2003
Accepted September 3, 2003
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
A Physiological Study on Growth and Dibenzothiophene (DBT) Desulfurization Characteristics of Gordonia sp. CYKS1
1Dept. of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Daejeon 305-701, Korea 2Genetic Engineering Division, Korean Intellectual Property Office, Government Complex-Daejeon, Dunsan-dong, Daejeon Metropolitan City, 302-701, Korea 3Dept. of Environmental Science and Engineering, Ewha Womans University, 11-1, Daehyun-dong, Seodaemun-gu, Seoul 120-750, Korea 4Dept. of Chemical and Environmental Engineering, Soongsil University, 1-1, Sangdo-5 dong, Seoul 156-743, Korea
Korean Journal of Chemical Engineering, March 2004, 21(2), 436-441(6), 10.1007/BF02705433
Download PDF
Abstract
Physiological characteristics of DBT desulfurization and cell growth of Gordonia sp. CYKS1 were investigated. It exhibited a preference to ethanol in a medium containing two carbon sources, ethanol and one of the carbohydrates used, glucose, sucrose, maltose, and galactose although it consumed both carbon sources simultaneously. Cell growth on ethanol or glucose followed the Monod kinetics. The optimal range of pH for the desulfurization of DBT and the cell growth was 7 to 8. The desulfurization rate decreased about 30% at pH 6, and no significant desulfurization or cell growth was observed at pH 5. As the initial DBT concentration increased up to 1.5 mM, the desulfurization rate also increased while no significant changes in the growth rate were observed. The maximum desulfurization rate was 12.50 μmol L-1 h-1 at an initial DBT concentration of 1.5 mM. Cell growth and desulfurization activity were severely inhibited by the presence of 2-hydroxybiphenyl (2-HBP). When 0.05 mM of 2-HBP was added at the beginning, both of the desulfurization rate and cell growth rate decreased about 20%. It was found that cell growth and desulfurization were completely inhibited in the presence of 2-HBP at 0.15 mM or a higher concentration. The inhibition by 2,2'-dihydroxybiphenyl (DHBP) was less severe than 2-HBP. About 80% of desulfurization activity was retained in the presence of 2,2'-DHBP at 0.4 mM.
References
Chang JH, Rhee SK, Chang YK, Chang HN, Biotechnol. Prog., 14(6), 851 (1998)
Choi OK, Cho KS, Ryu HW, Chang YK, Biotechnol. Lett., 25(1), 73 (2003)
Denis-Larose C, Labbe D, Bergeron H, Jones AM, Greer CW, Al-Hawari J, Grossman MJ, Sankey BM, Lau PCK, Appl. Environ. Microbiol., 63(7), 2915 (1997)
Denome SA, Oldfield C, Nash LJ, Young KD, J. Bacteriol., 176, 6707 (1994)
Gallado ME, Fernadez A, Lorenzo VD, Garcia JL, Diaz E, J. Bacteriol., 179(22), 7156 (1997)
Gallagher JR, Olson ES, Stanley DC, FEMS Microbiol. Lett., 107, 31 (1993)
Gray KA, Pogrebinsky OS, Mrachko GT, Xi L, Monticello DJ, Squires CH, Nat. Biotechnol., 14, 1705 (1996)
Inoue J, Shaw JP, Rekik K, Harayama S, J. Bacteriol., 177, 1196 (1995)
Izumi Y, Ohshiro T, Ogino H, Hine Y, Shimao M, Appl. Environ. Microbiol., 60, 223 (1994)
Kayser KJ, Bielaga-Jones BA, Jackowski K, Odusan O, Kilbane JJ, J. Gen. Microbiol., 139, 3123 (1993)
Konishi J, Ishii Y, Onaka T, Okumura K, Suzuki M, Appl. Environ. Microbiol., 63, 3164 (1997)
Kropp KG, Andersson JT, Fedorak PM, Appl. Environ. Microbiol., 63(8), 3032 (1997)
Lee MK, Senius HD, Grossman MJ, Appl. Environ. Microbiol., 61(2), 4362 (1995)
Li MZ, Squires CH, Monticello DJ, Chids JD, J. Bacteriol., 178(22), 6409 (1996)
Nekodzuka S, Nakajimakambe T, Nomura N, Lu J, Nakahara T, Biocata. Biotrans., 15, 17 (1997)
Ohshiro T, Hine Y, Izumi Y, FEMS Microbiol. Lett., 118, 341 (1994)
Ohshiro T, Kobayashi Y, Hine Y, Izumi Y, Biosce. Biotech. Biochem., 59, 1349 (1995)
Ohshiro T, Suzuki K, Izumi Y, J. Ferment. Bioeng., 83(3), 233 (1997)
Ohshiro T, Suzuki K, Izumi Y, J. Ferment. Bioeng., 81(2), 121 (1996)
Omori T, Monna L, Saiki Y, Kodama T, Appl. Environ. Microbiol., 58, 911 (1992)
Pavel H, Forsman M, Shinger V, J. Bacteriol., 176, 7550 (1994)
Piddington CS, Kovacevich BR, Rambosek T, Appl. Environ. Microbiol., 61, 468 (1995)
Rhee SK, Chang JH, Chang YK, Chang HN, Appl. Environ. Microbiol., 64, 2327 (1998)
Setti L, Lanzarini G, Pifferi PG, "Immobilized Cells for Applications in Non-conventional Systems. Progress in Biotechnology 11 Immobilized Cells: Basics and Applications," R.H. Wijffels (ed.), Elsevier Science B.V., 777 (1996)
Setti L, Lanzarini G, Pifferi PG, Process Biochem., 30(8), 721 (1995)
Wang P, Krawiec S, Appl. Environ. Microbiol., 62, 1670 (1996)
Choi OK, Cho KS, Ryu HW, Chang YK, Biotechnol. Lett., 25(1), 73 (2003)
Denis-Larose C, Labbe D, Bergeron H, Jones AM, Greer CW, Al-Hawari J, Grossman MJ, Sankey BM, Lau PCK, Appl. Environ. Microbiol., 63(7), 2915 (1997)
Denome SA, Oldfield C, Nash LJ, Young KD, J. Bacteriol., 176, 6707 (1994)
Gallado ME, Fernadez A, Lorenzo VD, Garcia JL, Diaz E, J. Bacteriol., 179(22), 7156 (1997)
Gallagher JR, Olson ES, Stanley DC, FEMS Microbiol. Lett., 107, 31 (1993)
Gray KA, Pogrebinsky OS, Mrachko GT, Xi L, Monticello DJ, Squires CH, Nat. Biotechnol., 14, 1705 (1996)
Inoue J, Shaw JP, Rekik K, Harayama S, J. Bacteriol., 177, 1196 (1995)
Izumi Y, Ohshiro T, Ogino H, Hine Y, Shimao M, Appl. Environ. Microbiol., 60, 223 (1994)
Kayser KJ, Bielaga-Jones BA, Jackowski K, Odusan O, Kilbane JJ, J. Gen. Microbiol., 139, 3123 (1993)
Konishi J, Ishii Y, Onaka T, Okumura K, Suzuki M, Appl. Environ. Microbiol., 63, 3164 (1997)
Kropp KG, Andersson JT, Fedorak PM, Appl. Environ. Microbiol., 63(8), 3032 (1997)
Lee MK, Senius HD, Grossman MJ, Appl. Environ. Microbiol., 61(2), 4362 (1995)
Li MZ, Squires CH, Monticello DJ, Chids JD, J. Bacteriol., 178(22), 6409 (1996)
Nekodzuka S, Nakajimakambe T, Nomura N, Lu J, Nakahara T, Biocata. Biotrans., 15, 17 (1997)
Ohshiro T, Hine Y, Izumi Y, FEMS Microbiol. Lett., 118, 341 (1994)
Ohshiro T, Kobayashi Y, Hine Y, Izumi Y, Biosce. Biotech. Biochem., 59, 1349 (1995)
Ohshiro T, Suzuki K, Izumi Y, J. Ferment. Bioeng., 83(3), 233 (1997)
Ohshiro T, Suzuki K, Izumi Y, J. Ferment. Bioeng., 81(2), 121 (1996)
Omori T, Monna L, Saiki Y, Kodama T, Appl. Environ. Microbiol., 58, 911 (1992)
Pavel H, Forsman M, Shinger V, J. Bacteriol., 176, 7550 (1994)
Piddington CS, Kovacevich BR, Rambosek T, Appl. Environ. Microbiol., 61, 468 (1995)
Rhee SK, Chang JH, Chang YK, Chang HN, Appl. Environ. Microbiol., 64, 2327 (1998)
Setti L, Lanzarini G, Pifferi PG, "Immobilized Cells for Applications in Non-conventional Systems. Progress in Biotechnology 11 Immobilized Cells: Basics and Applications," R.H. Wijffels (ed.), Elsevier Science B.V., 777 (1996)
Setti L, Lanzarini G, Pifferi PG, Process Biochem., 30(8), 721 (1995)
Wang P, Krawiec S, Appl. Environ. Microbiol., 62, 1670 (1996)
