This paper involves a feasibility study on using molecular imprinted polymers as the sorbent materials in solid phase extraction for caffeine and theophylline from green tea. Two kinds of MIPs, with caffeine-theophylline mixture and pentoxifylline-theophylline mixture as the templates respectively, MAA as the monomer, EDMA as the crosslinker and AIBN as the initiator, were applied to this purpose. Mixture solution of caffeine and theophylline (1 μg/ml in acetonitrile) was applied to the solid extraction cartridges following a load, wash and elute procedure with acetonitrile, methanol, methanol-acetic acid (90/10, v/v) as the solvents, respectively. This solid phase extraction protocol was applied for extraction of caffeine and theophylline from green tea. Comparison between the results obtained with the MIPs cartridges and a traditional C18 reversed-phase cartridge was made. It showed that the MIP-based sorbent on the solid phase extraction was comparable with that of C₁₈ material. HPLC analysis using a C₁₈ column (5 μm, 250 × 4.6 mm from Rstech corporation), methanol: water (60 : 40, v/v) as the mobile phase at a flow rate of 0.6 ml/min was applied for the quantitative determination.

Molecular Imprinted Polymers Solid Phase Extraction Caffeine Theophylline

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