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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received May 18, 2004
Accepted June 21, 2004

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Development of Modified Stokes Expression to Model the Behavior of Expanded Beds Containing Polydisperse Resins for Protein Adsorption

Yong Rae Chae Yeo Joon Yoon¹ Keun Garp Ryu^†

School of Chemical Engineering and Bioengineering, University of Ulsan, Ulsan 680-749, Korea ¹Division of Nano Sciences and Department of Chemistry, Ewha Womans University, Seoul 120-750, Korea

Korean Journal of Chemical Engineering, September 2004, 21(5), 999-1002(4), 10.1007/BF02705584

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Abstract

Expanded bed behavior was modeled by using the Richardson-Zaki correlation between the superficial velocity of the feed stream and the void fraction of the bed. A polydisperse matterial, Chelating excellose^® (70-210 μm in diameter, 1.21 g/cm³ in density), which has Ni²⁺ ions for the selective binding of histidine-tagged proteins, was used as the resin. A method to modify the stokes expression to express the terminal settling velocity of the resins by introducing two empirical parameters, the effective diameter of the resins and an exponent for (P_p-P)/μ term, was developed. Combined use of the Richardson-Zaki correlation and the modified Stokes expression was successful in modeling the bed expansion by incorporating physical properties of feed streams and the resins.

Keywords

Expanded Bed Modeling Scale-up Stokes Expression

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