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Received December 15, 2004
Accepted March 2, 2005
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Degradation of Styrene by a New Isolate Pseudomonas putida SN1
Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Korea 1Department of Food Science and Technology, Kyungsung University, Busan 608-736, Korea
parksh@pusan.ac.kr
Korean Journal of Chemical Engineering, May 2005, 22(3), 418-424(7), 10.1007/BF02719421
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Abstract
Twelve styrene-utilizing bacteria were isolated from a biofilter used for treating gaseous styrene. A gramnegative strain had a high styrene-degrading activity and was identified as Pseudomonas putida SN1 by 16S rDNA analysis. The styrene degradation in SN1 was regarded to start with a monooxygenase enzyme which converted styrene to styrene oxide, a potentially important chiral building block in organic synthesis. SN1 could grow on styrene and styrene oxide, but not on benzene and toluene. The styrene degradation activity in SN1 was induced when incubated with styrene, and the induction was not inhibited by the presence of readily usable carbon sources such as glucose and citrate. The optimal activity was shown at pH 7.0 and 30 ℃ and estimated as 170 unit/g cell.
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References
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Cox HHJ, Faber BW, vanHeiningen WNM, Radhoe H, Doddema HJ, Harder W, Appl. Environ. Microbiol., 62, 1471 (1996)
Gennaro PD, Colmegna A, Galli E, Sello G, Pelizzoni F, Bestetti G, Appl. Environ. Microbiol., 65, 2794 (1999)
Grogan CR, Willertts A, Enzymatic, 3, 253 (1997)
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Hartmans S, Smits JP, vanderWerf MJ, Volkering F, deBont JAM, Appl. Environ. Microbiol., 55(11), 2850 (1989)
Hartmans S, vanderWerf MJ, deBont JAM, Appl. Environ. Microbiol., 56(5), 1347 (1990)
Hasegawa J, Ohashi T, Chem. Today, 44 (1996)
Hollmann F, Lin PC, Witholt B, Schmid A, J. Am. Chem. Soc., 125(27), 8209 (2003)
Itoh N, Yoshida K, Okada K, Biosci. Biotechnol. Biochem., 60(11), 1826 (1996)
Kang JM, Biological Degradation of Ethylene Chlorides using Methylosinus trichosporium OB3b, Thesis for master degree, Pusan National University (1998)
Kim JR, Biological Production of Hydrogen from Carbno Monoxide and Water by the Novel Chemoheterotrophic Bacterium Citrobacter sp. Y19, Thesis for master degree, Pusan National University (2000)
Kim SB, Yoon JH, Kim H, Lee ST, Park YH, Goodfellow M, Int. J. Syst. Bacteriol., 45, 351 (1995)
Klasson KT, Lundback KMO, Clausen EC, Gaddy JL, J. Biotechnol., 29, 177 (1993)
Marconi AM, Beltrametti F, Bestetti G, Solinas F, Ruzzi M, Galli E, Zennaro E, Appl. Environ. Microbiol., 62, 121 (1996)
Martinez-Blanco H, Reglero A, Rodriquez-Aparacio LB, Luengo JM, J. Biol. Chem., 265, 7084 (1990)
Nothe C, Hartmans S, Biocatalysis, 10, 219 (1994)
O'Connor KE, Dobson ADW, Hartmans S, Appl. Environ. Microbiol., 63, 4287 (1997)
O'Connor KE, Buckley CM, Hartmans S, Dobson DW, Appl. Environ. Microbiol., 61, 544 (1995)
O'Leary ND, O'conner KE, Duetz W, Dobson ADW, Microbiology, 147, 973 (2001)
Panke S, deLorenzo V, Kaiser A, Witholt B, Wubbolts MG, Appl. Environ. Microbiol., 65, 5619 (1999)
Panke S, Witholt B, Schmid A, Wubbolts M, Appl. Environ. Microbiol., 64(6), 2032 (1998)
Sambrook J, Fritsch EF, Maniatis T, Molecular Cloning: A Laboratory Manual, 2nd edition, Gold Spring Harbor Laboratory Press, NY (1989)
Santos PM, Blatny JM, deBartolo I, Valla S, Zennaro E, Appl. Environ. Microbiol., 66(4), 1305 (2000)
Sheldon RA, Chemtech. September, 566, 576 (1991)
Shiray K, Hisatsuka K, Agric. Biol. Chem., 43, 1595 (1979)
Shiray K, Hisatsuka K, Agric. Biol. Chem., 43, 1399 (1979)
Velasco A, Alonso S, Garcia JL, Perera J, Diaz E, J. Bacteriol., 180(5), 1063 (1998)
Warhurst AM, Fewson CA, J. Appl. Bacteriol., 77, 597 (1994)
Wubbolts MG, Reuvekamp P, Witholt B, Enzyme Microb. Technol., 16(7), 608 (1994)
Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M, Microbiol. Immunol., 36, 1251 (1992)
Yoo SS, Production of (S)-Styrene Oxide by Microbial Enantioselective Hydrolysis, Thesis for masters degree, Pusan National University (2003)
Yeom SH, Yoo YJ, Korean J. Chem. Eng., 19(5), 780 (2002)