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In relation to this article, we declare that there is no conflict of interest.
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Received December 15, 2004
Accepted March 2, 2005
articles This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Degradation of Styrene by a New Isolate Pseudomonas putida SN1

Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735, Korea 1Department of Food Science and Technology, Kyungsung University, Busan 608-736, Korea
parksh@pusan.ac.kr
Korean Journal of Chemical Engineering, May 2005, 22(3), 418-424(7), 10.1007/BF02719421
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Abstract

Twelve styrene-utilizing bacteria were isolated from a biofilter used for treating gaseous styrene. A gramnegative strain had a high styrene-degrading activity and was identified as Pseudomonas putida SN1 by 16S rDNA analysis. The styrene degradation in SN1 was regarded to start with a monooxygenase enzyme which converted styrene to styrene oxide, a potentially important chiral building block in organic synthesis. SN1 could grow on styrene and styrene oxide, but not on benzene and toluene. The styrene degradation activity in SN1 was induced when incubated with styrene, and the induction was not inhibited by the presence of readily usable carbon sources such as glucose and citrate. The optimal activity was shown at pH 7.0 and 30 ℃ and estimated as 170 unit/g cell.

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