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Received December 27, 2004
Accepted April 19, 2005
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Mixing and Enzyme Reactions in a Microchannel Packed with Glass Beads
Department of Chemical Science and Technology, The University of Tokushima, 2-1 Minami Josanjimacho, Tokushima, 770-8506, Japan 1Department of Applied Chemistry, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan 2Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Japan 3Department of Living Environmental Science, Fukuoka Women’s University, 1-1-1 Kasumigaoka, Higashi-ku, Fukuoka 813-8529, Japan
sotowa@chem.tokushima-u.ac.jp
Korean Journal of Chemical Engineering, July 2005, 22(4), 552-555(4), 10.1007/BF02706641
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Abstract
.Mixing behavior in a microchannel was investigated by means of a spectrophotometer equipped with optical fiber probes. Microreactors with a Y-shaped channel were fabricated on poly(methyl methacrylate) substrates. Glass beads were packed in the intersection and the downstream regions to improve mixing performance. An NaOH solution and a BTB solution as a pH indicator were fed to the microchannel with a syringe pump. As the mixing progressed, the color of the mixed solution changed to blue. The degree of mixing was evaluated by the change in absorbance at 623 nm of the mixing solution. The packed glass beads strongly enhanced the mixing performance in the microchannels, especially in the case of packing in the intersection. The effect of mixing in the microchannel on an enzyme reaction was also investigated. The hydrolysis of o-nitrophenyl-β-D-galactopyranoside by β-galactosidase was used as a model reaction. The results showed that the enzyme reaction was enhanced in the microreactor with glass beads compared with a batch reactor. The microreactor packed with glass beads gave the highest reaction rate.
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