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Received April 11, 2005
Accepted June 30, 2005
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Monitoring of Bioventing Process for Diesel-Contaminated Soil by Dehydrogenase Activity, Microbial Counts and the Ratio of n-Alkane/Isoprenoid
Department of Environmental Engineering, Pusan National University, Busan 609-735, Korea 1POSCO Engineering & Construction Co., Ltd., Hwasung-Shi, Gyunggi-Do 445-810, Korea 2Department of Chemical Engineering, Pusan National University, Busan 609-735, Korea 3Department of Environmental Engineering, Andong National University, Andong 760-749, Korea
big815@pusan.ac.kr
Korean Journal of Chemical Engineering, November 2005, 22(6), 917-921(5), 10.1007/BF02705675
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Abstract
Monitoring parameters were evaluated for a bioventing process that was designed to treat soils contaminated with diesel fuel. Statistical analyses were conducted to evaluate correlations between total petroleum hydrocarbon concentrations in the contaminated soil and physico-chemical parameters of soil such as microbial counts, dehydrogenase activity, and n-alkane/isoprenoid ratio. The correlation coefficients (r2) obtained showed that TPH concentrations in the bioventing system were strongly correlated with dehydrogenase activity (DHA), total heterotrophic bacterial count, and hydrocarbon utilizing bacterial count. Thus, it was concluded that these parameters could useful monitoring parameters for soils contaminated with diesel fuel.
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References
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Balba MT, Al-Daher R, Al-Awadhi N, J. Microbiol. Methods, 32, 155 (1998)
Carter MR, Soil Sampling and Methods of Analysis, Lewis Publisher, U.S.A. (1993)
Dupont RR, Environ. Prog., 12, 45 (1993)
Hur JM, Park JA, Korean J. Chem. Eng., 20(2), 307 (2003)
Lesson A, Hichee RE, Principle and Practices of Bioventing: Volume I-bioventing Principles; Volume II-bioventing Design, National Risk Management Research Laboratory, U.S. EPA and U.S. Air Force Center for Environmental Excellence, Technology Transfer Division, Brooks AFB, Texas, 29 September (1995)
Margesin R, Schinner F, Appl. Microbiol. Biotechnol., 47(4), 462 (1997)
Margesin R, Zimmerbauer A, Schinner F, Chemosphere, 40, 339 (2000)
Nocentini M, Pinelli D, Fava F, Chemosphere, 41, 1115 (2000)
Noris RD, (Ed.), Handbook of Bioremediation, CRC press, Boca Raton, Florida (1994)
Page AL, Miller RH, Keeney DR, Methods of Soil Analysis, Part II, 2nd ed., ASA-SSSA, Wisconsin, 937-970 (1982)
Seklemova E, Pavlova A, Kovacheva K, Biodegradation, 12, 311 (2001)
Skujins J, "Extracellular Enzymes in Soil", CRC Crit. Rev. Microbiol., 4, 383 (1976)
US EPA, Bioventing Principle and Practice : Bioventing Principles, EPA, Washington, DC. (1995)
van Beelen PV, Doelman P, Chemosphere, 34, 455 (1997)
