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Received February 14, 2005
Accepted February 27, 2006
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Modification of lipase from Candida rugosa with poly(ethylene oxide-co-maleic anhydride) and its separation using aqueous two-phase partition system
Department of Chemical Engineering, Yonsei University, 134 Shinchon-dong, Seodaemoon-gu, Seoul 120-749, Korea
Korean Journal of Chemical Engineering, July 2006, 23(4), 601-606(6), 10.1007/BF02706801
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Abstract
A copolymer was synthesized from polyethylene oxide (PEO) and maleic acid anhydride (MA). Number of ethylene oxide units was varied from 10 to 40. Lipase from Candida rugosa was modified through chemical bonding of MA with amino group of lipase. Degree of modification increased with a decrease in EO unit and increase in copolymer/ enzyme ratio. The relative activity of modified enzyme increased with increase in EO unit. It was more than native lipase (100%) when copolymer/enzyme weight ratio was less than 3 for all copolymers. It might be due the conformation change of the lipase molecules on modification that would have exposed the catalytic sites making them more easily accessible. At the highest DM (39%), modified lipase retained more than 50% relative activity. Partitioning of native and modified lipase was also studied by using aqueous two phase synthesized copolymer/dextran system: modified lipase (with EO 30 and 40) showed better separation than the native one. Partition coefficient increased with increase in copolymer/enzyme weight ratio.
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References
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Chae HJ, In MJ, Kim EY, Appl. Biochem. Biotechnol., 73(2-3), 195 (1998)
Charusheela A, Arvind L, Enzyme Microb. Technol., 30(1), 19 (2002)
Cho SW, Rhee JS, Biotechnol. Bioeng., 41, 204 (1993)
Diamond AD, Hsu JT, Advances in Biochem. Eng. Biotech., 49, 89 (1992)
Dordick JS, Biocatalysis for industry, Plenum Press, New York (1991)
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Flory PJ, J. Chem. Phys., 9, 660 (1941)
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Furukawa M, Kodera Y, Uemura T, Hiroto M, Matsushima A, Kuno H, Matsushita H, Inada Y, Biochem. Biophys. Res. Commun., 199, 41 (1996)
Goto M, Kamiya N, Miyata M, Nakashio F, Biotechnol. Prog., 10(3), 263 (1994)
Zheng G, Shu B, Yan S, Enzyme Microb. Technol., 32(7), 776 (2003)
Guo Z, Sun Y, Biotechnol. Prog., 20(2), 500 (2004)
Habeeb AFSF, Anal. Biochem., 14, 328 (1966)
Huggins ML, J. Am. Chem. Soc., 64, 1712 (1942)
Hwang S, Lee KT, Park JW, Min BR, Haam S, Ahn IS, Jung JK, Biochem. Eng. J., 17, 85 (2004)
Jensen RG, Lipids, 18, 650 (1983)
Jeon GJ, Hur BK, Yang JW, Korean J. Biotechnol. Bioeng., 14, 696 (1999)
Jeong GT, Byun KY, Lee WT, Ryu HW, Sunwoo C, Kim HS, Park DH, “Enzymatic synthesis of sorbitan methacrylate: Comparison of methacrylic acid and vinyl methacrylate,” Biochem. Eng. J., In Press, Available online
Joo H, Yoo YJ, Dordick JS, Korean J. Chem. Eng., 15(4), 362 (1998)
Gee KB, Choi CY, Korean J. Chem. Eng., 1(1), 13 (1984)
Kim Y, Park K, Lee H, Jang S, Song HC, Shin HC, Park JJ, Park J, Maken S, J. Ind. Eng. Chem., 10(3), 384 (2004)
Koeller KM, Wong CH, Nature, 409, 232 (2001)
Kontkanen H, Tenkanen M, Fagerstrom R, Reinikainen T, J. Biotechnol., 108, 51 (2004)
Kwon DY, Rhee JS, Korean J. Chem. Eng., 1(2), 153 (1984)
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Moon HY, Kim SY, Park JW, Kajiuchi T, Korean J. Chem. Eng., 10(4), 235 (1993)
Nishio T, Takahashi K, Tsuzuki T, Yoshimoto T, Kodera Y, Matsushima A, Saito Y, Inada Y, J. Biotechnol., 8, 39 (1988)
Noel M, Combes D, J. Biotechnol., 102, 23 (2003)
Park CY, Ryu YW, Kim C, Korean J. Chem. Eng., 18(4), 475 (2001)
Park JW, Park KN, Biotechnol. Tech., 13, 49 (1999)
Park JW, Korean J. Chem. Eng., 12(5), 523 (1995)
Park J, Park K, Bioresour. Technol., 79(1), 91 (2001)
Park JW, Park KN, Song HC, Shin HC, J. Biotechnol., 93, 203 (2002)
Park K, Park J, Song H, Shin H, Park J, Ahn JS, Korean J. Chem. Eng., 19(2), 285 (2002)
Park SW, Kim YI, Chang KS, Kim SW, Process Biochem., 37/2, 153 (2001)
Reetz MT, Curr. Opin. Chem. Biol., 6, 145 (2002)
Salis A, Pinna M, Monduzzi M, Solinas V, J. Biotechnol., 119, 291 (2005)
Schmid A, Dordick JS, Hauer B, Wubbolts M, Witholt B, Nature, 409, 258 (2001)
Wang YH, Hsieh YL, J. Polym. Sci. A: Polym. Chem., 42(17), 4289 (2004)
Won K, Kim S, Kim KJ, Park HW, Moon SJ, Process Biochem., 40, 2149 (2005)
Wu JC, Song BD, Process Biochem., 37, 1229 (2002)
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Yadav GD, Lathi PS, Biochem. Eng. J., 16, 245 (2003)
Moon-Young Y, Lee SH, Cheong CS, Park JK, Enzyme Microb. Technol., 35(6-7), 574 (2004)
