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Received October 19, 2005
Accepted February 15, 2006
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Characteristics and recognition mechanism of monolithic poly(methacrylic acid-ethylene glycol dimethacrylate) column
1Center for Advanced Bioseparation Technology, Department of Chemical Engineering, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon 402-751, Korea 2Department of Chemistry, Baoding Teachers College, Baoding 071000, Korea
rowkho@inha.ac.kr
Korean Journal of Chemical Engineering, July 2006, 23(4), 625-630(6), 10.1007/BF02706805
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Abstract
Porous polymer monolithic columns have been prepared by the direct free radical copolymerization of methacrylic acid and ethylene glycol dimethacrylate within the confines of a chromatographic column in the presence of toluene-dodecanol as a porogenic solvent. Recognition mechanism and effects of the chromatographic condition such as mobile phase composition, flow rate and temperature on the retention and separation were discussed. The results showed hydrogen-bonding interaction and hydrophobic interaction play an important role in retention and separation in this kind of monolithic column. Compared with traditional particle columns, the monolithic columns showed attractive significant interest because of their ease of preparation, high separation efficiency, low backpressure and fast analysis.
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References
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