Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received February 27, 2006
Accepted May 16, 2006
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Ion-exchange properties of mildiomycin on HZ110TM resin
Pharmaceutical Informatics Institute, Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 310027, China 1Institute of Bioengineering, Department of Chemical Engineering and Bioengineering, Zhejiang University, Hangzhou 310027, China
Korean Journal of Chemical Engineering, November 2006, 23(6), 991-996(6), 10.1007/s11814-006-0019-x
Download PDF
Abstract
Mildiomycin (MIL) is a novel nucleoside agro-antibiotic which shows a specific and strong inhibitory activity against fungi, especially, powdery mildews. Because of its low action dosage, excellent environmental compatibility and remarkably low toxicity to human and animals, MIL is regarded as a kind of green biological pesticides. In this paper, the recovery and purification of MIL from fermentation broth were investigated by ion-exchange separation technique. The ion-exchange properties of MIL on HZ110TM resin, including ion-exchange equilibrium and dynamics in column process, were discussed. Results showed that the equilibrium exchange capacity of MIL was 26.5 mg per gram of wet resin at optimal pH value of pH 7.0. Ion-exchange isotherms of MIL can be well correlated with Freundlich equation. The ion-exchange capacity increased with raising the temperature. In the dynamic column process, breakthrough capacity was 29.3 mg per gram of wet resin. According to the ratio of desorption and eluent concentration of MIL, 2% ammonia aqueous solution was chosen as the eluent. And the total recovery ratio of MIL in ion-exchange process was up to 94.5%, and the enriched factor was about 8.
References
Chen Y, Xu ZN, Shen WH, Cen PL, Chinese J. Pestic., 43, 117 (2004)
Chen Y, Xu Z, Shen W, Lin J, Cen P, Korean J. Chem. Eng., 22(1), 121 (2005)
Harada S, Kishi T, J. Antibiot., 6, 519 (1978)
He BL, Huang WQ, Ion-exchange and adsorption resin, Shanghai Scientific and Technological Education Press, Shanghai (1995)
Kishimoto K, Akiyama S, Biotechnol. Lett., 19(7), 699 (1997)
Mujeebur RK, Wajid MK, Environ. Exp. Bot., 40, 265 (1998)
Reuveni M, Cohen H, Crop Prot,, 19, 393 (2000)
Kim SJ, Cho SY, Kim TY, Korean J. Chem. Eng., 19(1), 61 (2002)
Suzuki T, Sawada H, Method for producing mildiomycin, US patent, 4334022 (1982)
J. Pestic. Sci., Takeda Pharmaceutical Company Limited, “Summary of toxicological studies on mildiomycin,”, 19, 135 (1994)
Chen Y, Xu Z, Shen W, Lin J, Cen P, Korean J. Chem. Eng., 22(1), 121 (2005)
Harada S, Kishi T, J. Antibiot., 6, 519 (1978)
He BL, Huang WQ, Ion-exchange and adsorption resin, Shanghai Scientific and Technological Education Press, Shanghai (1995)
Kishimoto K, Akiyama S, Biotechnol. Lett., 19(7), 699 (1997)
Mujeebur RK, Wajid MK, Environ. Exp. Bot., 40, 265 (1998)
Reuveni M, Cohen H, Crop Prot,, 19, 393 (2000)
Kim SJ, Cho SY, Kim TY, Korean J. Chem. Eng., 19(1), 61 (2002)
Suzuki T, Sawada H, Method for producing mildiomycin, US patent, 4334022 (1982)
J. Pestic. Sci., Takeda Pharmaceutical Company Limited, “Summary of toxicological studies on mildiomycin,”, 19, 135 (1994)
