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Received July 11, 2006
Accepted September 6, 2006
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Fluorescence in situ hybridization and INT-dehydrogenase activity test to assess the effect of DO concentration in aerobic biofilm reactor
Department of Environmental Engineering, Pusan National University, Busan 609-735, Korea 1Institute for Environmental Technology and Industry, Pusan National University, Busan 609-735, Korea 2Department of Environmental Engineering, Andong National University, Andong 760-749, Korea
taejoo@pusan.ac.kr
Korean Journal of Chemical Engineering, January 2007, 24(1), 93-98(6), 10.1007/s11814-007-5015-2
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Abstract
To assess the effect of DO concentration in aerobic biofilm reactor, we investigated the bacterial communities and their activity utilizing the combination of two methods, fluorescence in situ hybridization (FISH) and INTdehydrogenase activity test. D-1, D-2, D-3 and D-4 reactor with different DO concentrations (1, 3, 5 and 7 mg/L, respectively) were set up in the thermostat and acclimated. The optimal DO concentration with stable nitrification efficiency in aerobic biofilm reactor was above 5.0 mg/L. FISH method showed us that higher DO concentration led to the increase of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria ratios and the decrease of heterotrophs ratio. The INT-DHA activities of each reactor were 203, 153, 36 and 45 mgO2*/g VSS/day, respectively. The INT-DHA activities in D-1 and D-2 reactors were higher than those of D-3 and D-4 reactors, which indicates that filamentous microorganisms affected the INT-DHA activity. In the case of D-3 and D-4 reactors, as nitrification efficiency increased, INT-DHA activity also increased. In the relationship between the attached biomass and nitrification efficiency, only active biomass influenced the nitrification efficiency.
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References
Awong J, Bitton G, Koopman B, Water Res., 19, 917 (1985)
Bitton G, Koopman B, Appl. Environ. Microbiol., 43, 964 (1982)
Daims H, Bruhl A, Amann R, Schleifer KH, Wagner M, Syst. Appl. Microbiol., 22, 434 (1999)
Daims H, Nielsen PH, Nielsen JL, Juretschko S, Wagner M, Water Sci. Technol., 41, 85 (2000)
Imat T, Kusuda T, Furumat H, Kinetic study and mathematical modeling of biofilm in an anaerobic fluidized bed, In Proc. 2nd Int. Specialized Conf. on Biofilm Reactors, Paris, France, 463-470 (1993)
Jang A, Bishop PL, Okabe S, Lee SG, Kim IS, Water Sci. Technol., 47, 49 (2002)
Kim CW, Koopman B, Bitton G, Water Res., 28, 1117 (1994)
Kim DJ, Ahn DH, Lee DI, Korean J. Chem. Eng., 22(1), 85 (2005)
Koopman B, Bitton G, Logue C, Bossart JM, Lopez JM, Validity of tetrazolium reduction assays for assessing toxic inhibition of filamentous bacteria in activated sludge, In Toxicity Screening Procedures using Bacterial Systems, 147-162, NY, USA (1984)
Lazarova NR, Manem J, Melo L, Water Sci. Technol., 37, 189 (1998)
Lazarova V, Pierzo V, Fontviell D, Manem J, Water Sci. Technol., 29, 345 (1994)
Liu Y, Capdeville B, Water Res., 30, 1645 (1996)
Mobarry BK, Wagner M, Urbain V, Rittmann E, Stahl DA, Appl. Environ. Microbiol., 62, 2156 (1996)
Park TJ, Lee KH, Kim DS, Kim CW, Water Sci. Technol., 34, 9 (1996)
Lee KH, Lee JH, Park TJ, Korean J. Chem. Eng., 15(1), 9 (1998)
Schramm A, De Beer D, Wagner M, Amann R, Appl. Environ. Microbiol., 64, 3480 (1998)
Sharrma B, Ahlert RC, Water Res., 11, 897 (1977)
Surmacz-Gorska J, Cichon A, Miksch K, Proceedings of the Env. Biotech., 1, 78 (1996)
Wagner M, Rath G, Koops HP, Flood J, Amann R, Water Sci. Technol., 34, 237 (1996)
Wang B, Yang O, Liu R, Yuan J, Ma F, He J, Li G, Water Sci. Technol., 24, 197 (1991)
Yang H, Jiang Z, Shi S, Tang WZ, Ecotoxicology and Environmental Safety, 53, 416 (2002)
Zhang TC, Bishop PL, Water Environ. Res., 68, 1107 (1996)
Bitton G, Koopman B, Appl. Environ. Microbiol., 43, 964 (1982)
Daims H, Bruhl A, Amann R, Schleifer KH, Wagner M, Syst. Appl. Microbiol., 22, 434 (1999)
Daims H, Nielsen PH, Nielsen JL, Juretschko S, Wagner M, Water Sci. Technol., 41, 85 (2000)
Imat T, Kusuda T, Furumat H, Kinetic study and mathematical modeling of biofilm in an anaerobic fluidized bed, In Proc. 2nd Int. Specialized Conf. on Biofilm Reactors, Paris, France, 463-470 (1993)
Jang A, Bishop PL, Okabe S, Lee SG, Kim IS, Water Sci. Technol., 47, 49 (2002)
Kim CW, Koopman B, Bitton G, Water Res., 28, 1117 (1994)
Kim DJ, Ahn DH, Lee DI, Korean J. Chem. Eng., 22(1), 85 (2005)
Koopman B, Bitton G, Logue C, Bossart JM, Lopez JM, Validity of tetrazolium reduction assays for assessing toxic inhibition of filamentous bacteria in activated sludge, In Toxicity Screening Procedures using Bacterial Systems, 147-162, NY, USA (1984)
Lazarova NR, Manem J, Melo L, Water Sci. Technol., 37, 189 (1998)
Lazarova V, Pierzo V, Fontviell D, Manem J, Water Sci. Technol., 29, 345 (1994)
Liu Y, Capdeville B, Water Res., 30, 1645 (1996)
Mobarry BK, Wagner M, Urbain V, Rittmann E, Stahl DA, Appl. Environ. Microbiol., 62, 2156 (1996)
Park TJ, Lee KH, Kim DS, Kim CW, Water Sci. Technol., 34, 9 (1996)
Lee KH, Lee JH, Park TJ, Korean J. Chem. Eng., 15(1), 9 (1998)
Schramm A, De Beer D, Wagner M, Amann R, Appl. Environ. Microbiol., 64, 3480 (1998)
Sharrma B, Ahlert RC, Water Res., 11, 897 (1977)
Surmacz-Gorska J, Cichon A, Miksch K, Proceedings of the Env. Biotech., 1, 78 (1996)
Wagner M, Rath G, Koops HP, Flood J, Amann R, Water Sci. Technol., 34, 237 (1996)
Wang B, Yang O, Liu R, Yuan J, Ma F, He J, Li G, Water Sci. Technol., 24, 197 (1991)
Yang H, Jiang Z, Shi S, Tang WZ, Ecotoxicology and Environmental Safety, 53, 416 (2002)
Zhang TC, Bishop PL, Water Environ. Res., 68, 1107 (1996)
