Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received October 13, 2006
Accepted October 23, 2006
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Production of bacterial cellulose by Gluconacetobacter hansenii using a novel bioreactor equipped with a spin filter
Department of Chemical Engineering, Kyungpook National University, Daegu 702-701, Korea 1Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea
parkjk@knu.ac.kr
Korean Journal of Chemical Engineering, March 2007, 24(2), 265-271(7), 10.1007/s11814-007-5058-4
Download PDF
Abstract
In order to improve bacterial cellulose (BC) production yield by increasing the cell density, a new fermentation system using a spin filter was developed and its performance characteristics were tested. Fermentations were carried out in a fermenter equipped with a 6 flat-blade turbine impeller and a spin filter consisting of a cylinder surrounded by stainless steel mesh and whose stainless steel bottom was attached to the agitator shaft. This new fermentation assembly was tested under different experimental conditions for BC production by Gluconacetobacter hansenii PJK. In periodical perfusion culture without pH control, the BC production and the total cell mass increased with the culture time to 3.07 and 5.65 g/L, respectively, at 140 h of cultivation. The BC production was also tested at adjusted pH and pH 5 was found optimum for maximum BC production. At pH 5, in periodical perfusion culture, the BC production and the total cell mass reached to 4.57 and 11.52 g/L, respectively, after 140 h of cultivation. This amount of BC production was 2.9 times higher than that obtained in a conventional jar fermenter. The productivity improved and was 0.044 g/L·h at 68 h of cultivation.
References
Delmer DP, Amor Y, Plant Cell, 7, 987 (1995)
Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryu M, J. Mater. Sci., 24, 3141 (1989)
Cannon RE, Anderson SM, Crit. Rev. Microbiol., 17, 435 (1991)
Embuscado ME, Marks JS, BeMiler JN, Food Hydrocolloids, 8, 419 (1994)
Klemm D, Schumann D, Udhard U, Marsch S, Prog. Polym. Sci, 26, 1561 (2001)
Fontana JD, De Souza AM, Fontana CK, Torriani IL, Moreschi JC, Gallotti BJ, De Souza SJ, Narcisco GP, Bichara JA, Farah LFX, Appl. Biochem. Biotechnol., 24/25, 253 (1990)
Vandamme EJ, De Baets S, Vanbaelen A, Joris K, De Wulf P, Polym. Degrad. Stabil., 59, 93 (1998)
Schramm M, Hestrin S, J. Gen. Microbiol., 11, 123 (1954)
Park JK, Park YH, Jung JY, Biotechnol. Bioprocess Eng., 8, 83 (2003)
Park JK, Jung JY, Park YH, Biotechnol. Lett., 25(24), 2055 (2003)
Park JK, Jung JY, Park YH, Biotechnol. Bioprocess Eng., 9, 383 (2004)
Jung JY, Park JK, Chang HN, Enzyme Microb. Technol., 37, 347 (2005)
Herbert D, in Continuous culture of microorganisms, Monograph No. 12, Society of Chemical Industries, London (1961)
Rogers PL, Lee KJ, Tribe DE, Process Biochem., 15, 7 (1980)
Cheryan M, Mehaia MA, Process Biochem., 19, 204 (1984)
Chaudhary DS, Vigneswaran S, Ngo HH, Shim WG, Moon H, Korean J. Chem. Eng., 20(6), 1054 (2003)
Kim DJ, Ahn DH, Lee DI, Korean J. Chem. Eng., 22(1), 85 (2005)
Margaritis A, Wilke CR, Biotechnol. Bioeng., 20, 727 (1978)
Valla S, Kjosbakken J, J. Gen. Microbiol., 128, 1401 (1981)
Gabe DR, J. Appl. Electrochem., 13, 3 (1983)
Du Toit WJ, Pretorius IS, Ann. Microbiol., 52, 155 (2002)
Chao Y, Mitarai M, Sugano Y, Shoda M, Biotechnol. Prog., 17, 781 (2001)
Ishida T, Mitarai M, Sugano Y, Shoda M, Biotechnol. Bioeng., 83(4), 474 (2003)
Coucheron DH, J. Bacteriol., 173, 5723 (1991)
Krystynowicz A, Koziol/kiewicz M, Wiktorowska-Jezierska A, Bielecki S, Klemenska E, Masny A, Plucienniczak A, Acta Biochim. Pol., 52, 691 (2005)
Yamanaka S, Watanabe K, Kitamura N, Iguchi M, Mitsuhashi S, Nishi Y, Uryu M, J. Mater. Sci., 24, 3141 (1989)
Cannon RE, Anderson SM, Crit. Rev. Microbiol., 17, 435 (1991)
Embuscado ME, Marks JS, BeMiler JN, Food Hydrocolloids, 8, 419 (1994)
Klemm D, Schumann D, Udhard U, Marsch S, Prog. Polym. Sci, 26, 1561 (2001)
Fontana JD, De Souza AM, Fontana CK, Torriani IL, Moreschi JC, Gallotti BJ, De Souza SJ, Narcisco GP, Bichara JA, Farah LFX, Appl. Biochem. Biotechnol., 24/25, 253 (1990)
Vandamme EJ, De Baets S, Vanbaelen A, Joris K, De Wulf P, Polym. Degrad. Stabil., 59, 93 (1998)
Schramm M, Hestrin S, J. Gen. Microbiol., 11, 123 (1954)
Park JK, Park YH, Jung JY, Biotechnol. Bioprocess Eng., 8, 83 (2003)
Park JK, Jung JY, Park YH, Biotechnol. Lett., 25(24), 2055 (2003)
Park JK, Jung JY, Park YH, Biotechnol. Bioprocess Eng., 9, 383 (2004)
Jung JY, Park JK, Chang HN, Enzyme Microb. Technol., 37, 347 (2005)
Herbert D, in Continuous culture of microorganisms, Monograph No. 12, Society of Chemical Industries, London (1961)
Rogers PL, Lee KJ, Tribe DE, Process Biochem., 15, 7 (1980)
Cheryan M, Mehaia MA, Process Biochem., 19, 204 (1984)
Chaudhary DS, Vigneswaran S, Ngo HH, Shim WG, Moon H, Korean J. Chem. Eng., 20(6), 1054 (2003)
Kim DJ, Ahn DH, Lee DI, Korean J. Chem. Eng., 22(1), 85 (2005)
Margaritis A, Wilke CR, Biotechnol. Bioeng., 20, 727 (1978)
Valla S, Kjosbakken J, J. Gen. Microbiol., 128, 1401 (1981)
Gabe DR, J. Appl. Electrochem., 13, 3 (1983)
Du Toit WJ, Pretorius IS, Ann. Microbiol., 52, 155 (2002)
Chao Y, Mitarai M, Sugano Y, Shoda M, Biotechnol. Prog., 17, 781 (2001)
Ishida T, Mitarai M, Sugano Y, Shoda M, Biotechnol. Bioeng., 83(4), 474 (2003)
Coucheron DH, J. Bacteriol., 173, 5723 (1991)
Krystynowicz A, Koziol/kiewicz M, Wiktorowska-Jezierska A, Bielecki S, Klemenska E, Masny A, Plucienniczak A, Acta Biochim. Pol., 52, 691 (2005)
