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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received September 15, 2006
Accepted November 17, 2006

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Homology search of genus pleurotus using an internal transcribed spacer region

Du Bok Choi Ji Lu Ding¹ Wol-Suk Cha^1†

Biotechnology Laboratory, B-K Company Ltd., 228 Soryong-dong, Gusan-si, Jeonlabuk-do 573-879, Korea ¹Department of Biochemical Engineering, Chosun University, Gwang-ju 501-759, Korea

wscha@mail.chosun.ac.kr

Korean Journal of Chemical Engineering, May 2007, 24(3), 408-412(5), 10.1007/s11814-007-0070-2

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Abstract

In order to establish the phylogenetic relationships of genus Pleurotus, internal transcribed spacer (ITS) regions of nine strains of mushrooms were amplified and sequenced. Fungi-Seq-F1 primer and fungi-Seq-R1 were perfectly matched with 2007 and 2935 kinds of fungi, respectively. These results show that these primers can be used not only in the types of mushroom used in this study, but also in DNA sequencing analysis of the ITS region of any other mushroom. A BLAST search using about 500 bp of the 5' terminus of ITS region was carried out. The observed homology between some mushrooms and the ITS region was 98-100%. In order to investigate these results, we searched the GenBank databases. At the time of our search, the ITS region of the mushrooms was unknown and could not be found in the results of our database search in GenBank. Therefore, whole DNA sequencing of ITS region of the mushroom is considered to be of critical significance in view of future phylogenetic analyses. Additionally, the sequences of four mushrooms were aligned and showed 95-98% of homology.

Keywords

Pleurotus ITS Region Homology

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