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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received October 18, 2006
Accepted January 8, 2007

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Binary protein adsorption to DEAE sepharose FF

Mohammad Reza Aboudzadeh^† Neda Aboudzadeh¹ Zhu Jiawen² Wu Bin²

Nuclear Research Center for Agriculture & Medicine, AEOI, P.O. Box 31585-4395, Karaj, Iran ¹Materials Science and Engineering Department, Iran University of Science and Technology (IUST), P.O. Box 16846-13114, Tehran, Iran ²Chemical Engineering Research Center, East China University of Science and Technology (ECUST), Shanghai, 200237, Korea

Korean Journal of Chemical Engineering, July 2007, 24(4), 641-647(7), 10.1007/s11814-007-0017-7

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Abstract

Equilibrium between ion exchangers and proteins is one of the most important factors in ion exchange chromatography. A model system was used to simulate the adsorption of human serum albumin (HSA) and ovalbumin (OVA) as a binary protein system to the DEAE Sepharose FF as an anion exchanger. Two models, one based on a competition between adsorbing molecules and the other a non competitive model have been compared to experimental results. Competitive adsorption was seen in experiments in which breakthrough curves and the profiles of adsorbed proteins in packed beds were determined. However, although the results for packed bed experiments were more closely predicted by the competitive model, some discrepancies were found, suggesting that when considering multicomponent protein adsorption to ion exchangers it may also be necessary to take account of factors such as the molecular size of adsorbing proteins and any potential inter protein interaction, which may hinder the development of a general model of multicomponent protein adsorption to ion exchangers.

Keywords

Ion Exchange Chromatography Protein Adsorption Langmuir Isotherm HSA OVA

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