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Received January 15, 2007
Accepted January 24, 2007
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Enhancement of secreted production of glucoamylase through fed-batch bioreactor culture of recombinant yeast harboring glucose-controllable SUC2 promoter
Department of Chemical Engineering, Pohang University of Sicence and Technology, Pohang 790-784, Korea 1Department of Environmental Engineering and Biotechnology, Myongji University, Yongin 449-728, Korea 2School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea
hjcha@pohang.ac.kr
Korean Journal of Chemical Engineering, September 2007, 24(5), 812-815(4), 10.1007/s11814-007-0046-2
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Abstract
Glucoamylase that hydrolyses starch to glucose is one of the important industrial enzymes for ethanol production industry. Therefore, genetic production of recombinant glucoamylase has been widely studied. Previously, we reported secreted production of Saccharomyces diastaticus-originated glucoamylase in Saccharomyces cerevisiase expression system using its own signal sequence and the SUC2 promoter that is regulated by glucose level in culture medium. In the present work, we performed a comparative study between batch and fed-batch bioreactor cultures for secreted production of recombinant glucoamylase. Through maintaining low glucose levels in the culture broth, we obtained about 7-fold higher secreted production levels of glucoamlyase in fed-batch culture. Fed-batch culture strategy also enhanced (~3.1-fold) secretion efficiency of recombinant glucoamylase in S. cerevisiae.
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