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Received February 24, 2007
Accepted April 17, 2007
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Long-term preservation of high initial bioluminescence of lyophilized Photobacterium phosphoreum: Effect of skim milk and saccharose at various temperatures
Thuan-Hieu Tran1
Woo-Jin Chang2
Young-Bum Kim1
Joo-Young Yoon3
Yoon-Mo Koo1 2
Eun-Ki Kim1†
Jin-Hwan Kim4
1Department of Biological Engineering, Inha University, Incheon 402-751, Korea 2ERC for Advanced Bioseparation Technology, Inha University, Incheon 402-751, Korea 3M&B GreenUs Co., Ltd., Seoul 152-021, Korea 4Department of Polymer Science and Engineering, Polymer Technology Institute, Sungkyunkwan University, Suwon, Gyunggi-do 440-746, Korea
ekkim@inha.ac.kr
Korean Journal of Chemical Engineering, November 2007, 24(6), 1053-1057(5), 10.1007/s11814-007-0120-9
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Abstract
A lyophilization method for long-term preservation of the initial bioluminescence of Photobacterium phosphoreum was investigated. The initial bioluminescence of lyophilized P. phosphoreum at different temperatures, .20 ℃, 4 ℃, room temperature (16-25 ℃) and 45 ℃, was monitored for 6 months with different additives. Saccharose, skim milk, and a mixture of saccharose and skim milk were tested. Skim milk showed the best protection for bioluminescence among the additives used. The initial bioluminescence remained high during 6 months at a wide range of storage temperatures, i.e., .20 ℃, 4 ℃, and room temperature, with skim milk as an additive. The average bioluminescence of lyophilized P. phosphoreum using skim milk as additive reached 33392 RLU within 30 min of regeneration. Bioluminescence increased slightly after 10 h of incubation.
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