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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received February 15, 2007
Accepted May 30, 2007

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Purification of homoharringtonine and removal of residual solvents by spray drying

Byung-Sik Kim Jin-Hyun Kim^†

Department of Chemical Engineering, Kongju National University, 182, Shinkwan-dong, Kongju, Chungnam 314-701, Korea

Korean Journal of Chemical Engineering, January 2008, 25(1), 108-111(4), 10.1007/s11814-008-0019-0

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Abstract

Homoharringtonine was purified from Cephalotaxus koreana by a combination of extraction, synthetic adsorbent treatment, low-pressure chromatography, and high performance liquid chromatography (HPLC). A crude extract was obtained by methanol extraction of biomass, followed by liquid-liquid extraction using chloroform. The waxy compounds were efficiently removed by adsorbent (active clay P-1) treatment. The extract was purified to greater than 52% with an 86.4% step yield by silica gel low-pressure chromatography. High performance liquid chromatography steps, which were composed of an HPLC step with silica column and an HPLC step with ODS column, were applied to give 98% purity with high yield. Amorphous homoharringtonine, with a fine particle size, was simply made by dissolving homoharringtonine in methylene chloride/methanol (98/2, v/v), followed by spray drying. Residual solvents, methylene chloride and methanol, could be reduced to 250 ppm and 1,160 ppm by spray drying and successive drying in a vacuum oven.

Keywords

Cephalotaxus koreana Homoharringtonine (HHT) Purification Residual Solvents Spray Drying

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