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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 11, 2007
Accepted September 4, 2007

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of nitrogen sources on toluene degradation by Pseudomonas putida BZ918

Eung Sup Lee Jae Yeon Park Sung Ho Yeom¹ Young Je Yoo^†

School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea ¹Department of Environmental and Applied Chemical Engineering, Kangnung National University, Gangneung, Gangwon-do 210-702, Korea

Korean Journal of Chemical Engineering, January 2008, 25(1), 139-143(5), 10.1007/s11814-008-0025-2

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Abstract

For effective toluene degradation, the effects of a nitrogen source were studied with Pseudomonas putida BZ912, which was isolated from crude oil contaminated soil and is capable of degrading VOC. Two nitrogen sources, ammonia and nitrate, showed different effects on specific growth rates (0.25 hr.1 and 0.12 hr.1, respectively), biomass yields (0.56 vs. 0.39) and specific toluene degradation rates (0.51 hr.1 vs. 0.26 hr.1). Under the resting cell conditions, the cells pre-cultured in the ammonia-containing medium showed higher specific toluene degradation rate than that in nitrate-containing medium (0.045 hr.1 vs. 0.038 hr.1). Ammonia as a nitrogen source was effective for degradation in high toluene concentration because high cellular biomass was accomplished. Nitrate showed slow growth rate compared to ammonia. The resting cell conditions demonstrated that it was able to degrade toluene efficiently without increasing biomass. These conditions could be a solution for degrading VOC after high cellular biomass was obtained in a biofilter. By changing the nitrogen source and the growth conditions according to the toluene concentration, the control of cell biomass and the desired removal capacity were accomplished.

Keywords

Toluene Degradation Pseudomonas Ammonia Nitrate Biofilter VOC

References

Yeom SH, Daugulis AJ, Biotechnol. Bioeng., 72, 156 (2000)
Rittmann BE, McCarty PL, Environmental biotechnology: Principles and applications, McGraw-Hill, New York, pp. 721-722 (2001)
Hodge DS, Devinny JS, J. Enrion. Eng., 121, 21 (1995)
Park HS, Final reports of service research: Development of a guiding principle in estimating discharges of volatile organic compounds (VOCs), Ministry of Environment, Korea (2003)
Campbell HJ, Connor MA, Proceedings for the Environmental Biotechnology, 1, 279 (1996)
Jung IG, Park OH, Woo HJ, Park CH, Biotechnol. Bioproc. Eng., 10, 34 (2005)
Cox HHJ, Deshusses MA, Water Res., 33, 2383 (1999)
Weber FJ, Hartmans S, Biotechnol. Bioeng., 50(1), 91 (1996)
Yeom SH, Yoo YJ, Process Biochem., 34(3), 281 (1999)
Clesceri LS, Greengerg AE, Trussell RR, Standard methods for the examination of water and wastewater, 17th ed., American Public Health Association, pp. 120-121 (1989)
Madigan MT, Martinko JM, Paker J, Biology of microorganisms, 9th ed., Prentice-Hall, Inc., Upper Saddle River, NJ, pp. 102-133 (2000)
Schonduve P, Sara M, Friedl A, Appl. Microbiol. Biotechnol., 45(1-2), 286 (1996)
Rojas A, Duque E, Mosqueda G, Golden G, Hurtado A, Ramos JL, Segura A, J. Bacteriol., 183, 3967 (2001)
Shareefdeen Z, Baltzis BC, Chem. Eng. Sci., 49(24), 4347 (1994)
Tang HM, Hwang SJ, Hwang SC, Hazard. Waste. Hazard. Mater., 12, 207 (1995)
Kiared K, Fundenberger B, Brzezinski R, Viel G, Heitz M, Ind. Eng. Chem. Res., 36(11), 4719 (1997)
Yeom SH, Process Biochem., 42, 554 (2007)
Stanley NR, Lazazzera BA, Mol. Microbiol., 52, 917 (2004)