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Received June 8, 2007
Accepted July 11, 2007
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Enhanced stability of Candida antarctica lipase B in ionic liquids
Sung Ho Ha1
Sang Hyun Lee1
Dung Thanh Dang2
Min Sik Kwon2
Woo-Jin Chang1
Yong Jae Yu3
Il Suk Byun3
Yoon-Mo Koo1 2†
1ERC for Advanced Bioseparation Technology, Korea 2Department of Biological Engineering, Inha University, Incheon 402-751, Korea 3C-TRI, Hwaseong-city, Gyeonggi-do 445-743, Korea
Korean Journal of Chemical Engineering, March 2008, 25(2), 291-294(4), 10.1007/s11814-008-0051-0
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Abstract
The activity and stability of lipase from Candida antarctica were investigated in the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate using ionic liquids (ILs) as reaction media. Among ILs tested, the highest activity of lipase was observed in [Edmim][Tf2N]. In hydrophobic ILs such as [Edmim][Tf2N], [Emim][Tf2N] and [Pmim] [PF6], lipase could retain its activity after 5 times reuse, while the activity of lipase in hydrophilic ILs and organic solvents was drastically decreased. The activities of lipase in [Edmim][Tf2N], [Emim][Tf2N] and [Pmim][PF6] were also well maintained after 1 day incubation at 80 ℃. The lipase suspended in [Edmim][Tf2N] could be successfully reused 6 times without loss of activity.
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