Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received May 9, 2007
Accepted September 17, 2007
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Enhanced β-carotene production by Rhodotorula glutinis using high hydrostatic pressure
1Department of Food Science and Safety, China Pharmaceutical University, Nanjing 210009, Jiangsu, China 2College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 3Department of Food Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, China
cpuwsl@126.com
Korean Journal of Chemical Engineering, May 2008, 25(3), 513-516(4), 10.1007/s11814-008-0086-2
Download PDF
Abstract
High hydrostatic pressure (HHP) technology was used for improving the ability of β-carotene biosynthesis of Rhodotorula glutinis R68. After the treatments of five repeated cycles at 300MPa for 15 min, the barotolerant mutant PR68 was obtained. After 72 h of culture, the biomass of mutant PR68 was 21.6 g/L, decreased by 8.5% compared to the parent strain R68, but its β-carotene production reached 19.4 mg/L, increased by 52.8% compared to the parent strain R68. The result of restriction fragment length polymorphism analysis suggested that mutant strain PR68 was likely to change in nucleic acid level, and thus enhanced β-carotene production in this strain was a result of gene mutation induced by HHP treatment. HHP technology seems a promising approach for improving industrial microbes.
References
Bhosale PB, Gadre RV, Appl. Environ. Microbiol., 55, 423 (2001)
Simova ED, Frengova GI, Beshkova DM, J. Ind. Microbiol. Biotechnol., 31, 115 (2004)
Perrier V, Dubreucq E, Galzy P, Arch. Microbiol., 164, 173 (1995)
Bartlett DH, Biochim. Biophys. Acta, 1595, 367 (2002)
Ritz M, Tholozan JL, Federighi M, Pilet MF, Int. J. of Food Microbiol., 79, 47 (2002)
Basak S, Ramaswamya HS, Piette JPG, Innov. Food Sci. Emerg. Technol., 3, 223 (2002)
Bartlett DH, Kato C, Horikoshi K, Res. Microbiol., 146, 697 (1995)
Gao X, Li J, Ruan KC, Acta Biochim. Biophys. Sinica, 33, 77 (2001)
Hauben KJA, Bartlett DH, Soontjens CCF, Appl. Environ. Microbiol., 63, 945 (1997)
Wang SL, Wu XZ, Sun JS, Acta Microbiol. Sinica, 45, 970 (2005)
Wang SL, Wu XZ, Sun JS, Ind. Microbiol. Sinica, 36, 31 (2006)
Bartlett DH, Sci. Prog., 76, 479 (1992)
Wuytack EY, Diels AMJ, Michiels CW, Int. J. of Food Microbiol., 77, 205 (2002)
Qiao CS, Liu BN, Xu X, Jia SR, China Biotechnol., 26, 56 (2006)
Lee TC, Rodriguez DB, Karasawa I, Appl. Microbiol., 30, 988 (1975)
Simova ED, Frengova GI, Beshkova DM, J. Ind. Microbiol. Biotechnol., 31, 115 (2004)
Perrier V, Dubreucq E, Galzy P, Arch. Microbiol., 164, 173 (1995)
Bartlett DH, Biochim. Biophys. Acta, 1595, 367 (2002)
Ritz M, Tholozan JL, Federighi M, Pilet MF, Int. J. of Food Microbiol., 79, 47 (2002)
Basak S, Ramaswamya HS, Piette JPG, Innov. Food Sci. Emerg. Technol., 3, 223 (2002)
Bartlett DH, Kato C, Horikoshi K, Res. Microbiol., 146, 697 (1995)
Gao X, Li J, Ruan KC, Acta Biochim. Biophys. Sinica, 33, 77 (2001)
Hauben KJA, Bartlett DH, Soontjens CCF, Appl. Environ. Microbiol., 63, 945 (1997)
Wang SL, Wu XZ, Sun JS, Acta Microbiol. Sinica, 45, 970 (2005)
Wang SL, Wu XZ, Sun JS, Ind. Microbiol. Sinica, 36, 31 (2006)
Bartlett DH, Sci. Prog., 76, 479 (1992)
Wuytack EY, Diels AMJ, Michiels CW, Int. J. of Food Microbiol., 77, 205 (2002)
Qiao CS, Liu BN, Xu X, Jia SR, China Biotechnol., 26, 56 (2006)
Lee TC, Rodriguez DB, Karasawa I, Appl. Microbiol., 30, 988 (1975)
