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Received July 30, 2007
Accepted September 18, 2007
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Characterization of a carbon composite electrode for an electrochemical immunosensor
Department of Chemical and Biomolecular Engineering and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea 1Department of Chemical Engineering, University of Ulsan, Ulsan 680-749, Korea
koo@sogang.ac.kr
Korean Journal of Chemical Engineering, May 2008, 25(3), 548-552(5), 10.1007/s11814-008-0092-4
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Abstract
A bioactive platform with a carbon composite electrode was developed for rapid detection of Escherichia coli O157:H7. The porous carbon composite electrode was prepared by a sol-gel method with a mixture of graphite powder and tetraethyl orthosilicate/ethanol. Escherichia coli O157:H7 antibodies were physically adsorbed onto the carbon composite electrode. Direct measurements by cyclic voltammetry and electrochemical impedance spectroscopy in the presence of [Fe(CN)6]3-/4- as a redox probe showed that the immobilization of antibodies onto the carbon composite electrode surface and the binding of Escherichia coli O157:H7 cells with antibodies systematically increased the electron-transfer resistance. Those results suggest that a sol-gel derived graphite composite electrode might be utilized as a label-free electrochemical immunosensor for diagnosis, biochemical research, food industry, and so on.
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References
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Lee W, Park KS, Kim YW, Lee YH, Choi JW, Biosens. Bioelectron., 20, 2292 (2005)
Chuanmin R, Kefeng Z, Oomman KV, Craig AG, Anal. Chem., 75, 6494 (2003)
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