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Received October 2, 2007
Accepted November 30, 2007
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Immobilization of α-amylase from Bacillus licheniformis on developed support using microbial transglutaminase
1Department of Bioscience and Biotechnology, College of Engineering, Silla University, Gwaebop-dong 1-1, Busan 617-736, Korea 2Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University, Gwaebop-dong 1-1, Busan 617-736, Korea
jhalee@silla.ac.kr
Korean Journal of Chemical Engineering, July 2008, 25(4), 801-803(3), 10.1007/s11814-008-0131-1
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Abstract
α-amylase from Bacillus licheniformis was successfully immobilized on developed support, which was prepared by coating a chitosan-casein film on silica, at 20 ℃, pH 6.0 for 5 hr with microbial transglutaminase (MTG) as the cross-linking factor. The optimal support was obtained when 1% chitosan and 1% casein were used in the coating mixture. The optimal condition for immobilization catalyzed by MTG was confined to be at MTG concentration of 15 U/mL, pH 6.0, reacting for 6 hr at 20 ℃. The highest specific activity of immobilized α-amylase was achieved as 236 U/g. After immobilization, the obtained enzyme showed broader pH profile and maintained more than 70% of the original activity after 20 reuses.
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lkram-ul-Haq, Ashraf H, Qadeer MA, Iqbal J, Bioresour. Technol., 96(10), 1201 (2005)
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Kang SC, Kim HJ, Nam SW, Oh DK, J. Microbiol. Biotechnol., 5, 766 (2002)
