Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received November 28, 2007
Accepted February 27, 2008
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Multiple-copy-gene integration on chromosome of Escherichia coli for beta-galactosidase production
Institute of Biotechnology, Department of Chemical Engineering, National Taipei University of Technology, No.1, Sec. 3, Chung-Hsiao E. Rd, Taipei 10608, Taiwan
f10955@ntut.edu.tw
Korean Journal of Chemical Engineering, September 2008, 25(5), 1082-1087(6), 10.1007/s11814-008-0177-0
Download PDF
Abstract
Recombinant E. coli strains with 1-3 copies of lacZ genes on their chromosomes were constructed and their β-galactosidase (β-gal) expressions were examined. Serial dilution cultures were used to analyze the long-term genetic stability of the recombinant lacZ genes of the chromosomal or plasmid expression system. The strain with a 3-copy lacZ on the chromosome has a sustainable β-gal expression through 60 hours. However, the β-gal activity of_x000D_
the plasmid expression system lasted less than 36 hours under a no selection condition. bviously, the genetic stability of the chromosomal expression system demonstrated in this study is better than that of the plasmid expression system under nonselective condition, such as a medium without antibiotics. The results demonstrated that the strains with a multiple-copy-gene on the chromosome are useful for protein production in industrial repeated fed-batch fermentation.
References
Zhang Y, Buchholz F, Muyrers JP, Stewart AF, Nature Genet, 20, 123 (1998)
Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW, Cell, 33, 25 (1983)
Werten MW, van den Bosch TJ, Wind RD, Mooibroek H, Mooibroek FA, Yeast, 15, 1087 (1999)
Cha HJ, Yoo YJ, Korean J. Chem. Eng., 13(2), 172 (1996)
Srinivasan S, Barnard GC, Gerngross TU, Biotechnol. Bioeng., 84(1), 114 (2003)
Shin PK, Koo JH, Lee WJ, Seo JH, Korean J. Chem. Eng., 13(1), 82 (1996)
Yoo HJ, Seo JH, Kang DG, Cha HJ, Korean J. Chem. Eng., 24(1), 99 (2007)
Sun Z, Cai J, Korean J. Chem. Eng., 23(4), 607 (2006)
El Karoui M, Amundsen SK, Dabert P, Gruss A, Nucleic Acids Res., 27, 1296 (1999)
Wackernagel W, Biochem. Biophys. Res. Commun., 51, 306 (1973)
Yu D, Ellis HM, Lee EC, Court DL, Proc. Natl. Acad. Sci. USA, 97, 5978 (2000)
Datsenko KA, Wanner BL, Court DL, Proc. Natl. Acad. Sci. USA, 97, 6640 (2000)
Murphy KC, Campellone KG, BMC Mol. Bio., 4, 4 (2003)
Ito T, Sota H, Honda H, Shimizu K, Kobayashi T, Appl. Microbiol. Biotechnol., 36, 295 (1991)
Sambrook J, Fritsch EF, Maniatis T, Molecular cloning: A laboratory manual, Cold Spring Harbor Press, Cold Spring Harbor (1989)
Hahn SK, Ryu HW, Change YK, Korean J. Chem. Eng., 15(1), 51 (1998)
Miller JH, Experiments in molecular genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor (1972)
Koo YM, Parekh BS, Hatfield GW, Lim HC, Korean J. Chem. Eng., 13(2), 202 (1996)
Hou SY, Lai PR, Chen JC, Cheng HC, J. Chin. Inst. Chem. Eng., 36, 603 (2005)
Murphy KC, J. Bacteriol., 180, 2063 (1993)
Khlebnikov A, Datsenko KA, Skaug T, Wanner BL, Keasling JD, Microbiology, 147, 3241 (2001)
Kang Y, Durfee T, Glasner JD, Qiu Y, Frisch D, Winterberg KM, Blattner FR, J. Bacteriol., 186, 4921 (2004)
Chang TS, Wu WJ, Wan HM, Shin TR, Wu WT, Appl. Microbiol. Biotechnol., 61(3), 234 (2003)
Liao JC, Hou SY, Chao YP, Biotechnol. Bioeng., 52(1), 129 (1996)
Epstein W, Curtis SJ, J. Bacteriol., 122, 1189 (1975)
Metcalf WW, Wanner BL, Gene, 129, 17 (1993)
Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW, Cell, 33, 25 (1983)
Werten MW, van den Bosch TJ, Wind RD, Mooibroek H, Mooibroek FA, Yeast, 15, 1087 (1999)
Cha HJ, Yoo YJ, Korean J. Chem. Eng., 13(2), 172 (1996)
Srinivasan S, Barnard GC, Gerngross TU, Biotechnol. Bioeng., 84(1), 114 (2003)
Shin PK, Koo JH, Lee WJ, Seo JH, Korean J. Chem. Eng., 13(1), 82 (1996)
Yoo HJ, Seo JH, Kang DG, Cha HJ, Korean J. Chem. Eng., 24(1), 99 (2007)
Sun Z, Cai J, Korean J. Chem. Eng., 23(4), 607 (2006)
El Karoui M, Amundsen SK, Dabert P, Gruss A, Nucleic Acids Res., 27, 1296 (1999)
Wackernagel W, Biochem. Biophys. Res. Commun., 51, 306 (1973)
Yu D, Ellis HM, Lee EC, Court DL, Proc. Natl. Acad. Sci. USA, 97, 5978 (2000)
Datsenko KA, Wanner BL, Court DL, Proc. Natl. Acad. Sci. USA, 97, 6640 (2000)
Murphy KC, Campellone KG, BMC Mol. Bio., 4, 4 (2003)
Ito T, Sota H, Honda H, Shimizu K, Kobayashi T, Appl. Microbiol. Biotechnol., 36, 295 (1991)
Sambrook J, Fritsch EF, Maniatis T, Molecular cloning: A laboratory manual, Cold Spring Harbor Press, Cold Spring Harbor (1989)
Hahn SK, Ryu HW, Change YK, Korean J. Chem. Eng., 15(1), 51 (1998)
Miller JH, Experiments in molecular genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor (1972)
Koo YM, Parekh BS, Hatfield GW, Lim HC, Korean J. Chem. Eng., 13(2), 202 (1996)
Hou SY, Lai PR, Chen JC, Cheng HC, J. Chin. Inst. Chem. Eng., 36, 603 (2005)
Murphy KC, J. Bacteriol., 180, 2063 (1993)
Khlebnikov A, Datsenko KA, Skaug T, Wanner BL, Keasling JD, Microbiology, 147, 3241 (2001)
Kang Y, Durfee T, Glasner JD, Qiu Y, Frisch D, Winterberg KM, Blattner FR, J. Bacteriol., 186, 4921 (2004)
Chang TS, Wu WJ, Wan HM, Shin TR, Wu WT, Appl. Microbiol. Biotechnol., 61(3), 234 (2003)
Liao JC, Hou SY, Chao YP, Biotechnol. Bioeng., 52(1), 129 (1996)
Epstein W, Curtis SJ, J. Bacteriol., 122, 1189 (1975)
Metcalf WW, Wanner BL, Gene, 129, 17 (1993)
