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Received December 3, 2007
Accepted February 27, 2008
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Xylitol fermentation by Candida subtropicalis WF79 immobilized in polyacrylic hydrogel films
Department of Chemical Engineering, National Yunlin University of Science and Technology, 123, Section 3, University Road, Touliu, Yunlin 640, Taiwan, R.O.C. 1Jin Wen Institute of Technology, 99, An-Chung Road, Shin-Tien, Taipei County, 231, Taiwan, R.O.C. 2Department of Applied Chemistry, Chaoyang University of Technology, 168, Gifeng E. Rd., Wufeng, Taichung County, 413, Taiwan, R.O.C.
Korean Journal of Chemical Engineering, September 2008, 25(5), 1088-1093(6), 10.1007/s11814-008-0178-z
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Abstract
Polyacrylate hydrogel films were used as immobilization matrices for cell entrapment of the yeast strain, Candida subtropicalis WF79, isolated from sugarcane bagasse. The hydrogel films with immobilized viable yeast cells were employed for the bioconversion from xylose to xylitol. The hydrogel was prepared from the monomers of 2-hydroxyethyl methacrylate (HEMA), methacrylic acid (MAA), and N,N-dimethyl acrylamide (DMA) with polyethylene glycol diacrylate (PEG-DA, weight-average molecular weight Mw=400 and 1,000 g/mol) as the crosslinking agent. The mechanical properties and fermentation yields of the immobilized polyacrylate hydrogel film with different monomeric formulation were investigated. Fermentation was carried out in a medium of 100 mL aqueous solution containing various amount of xylose. The conversion rate of xylose to xylitol conversion reached a maximum value of 80% after 120 h. The bioconversion activity gradually declined, after 720 h, to 65% conversion at 1,080 h.
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References
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Silva SS, Afschar AS, Bioprocess Eng., 11, 129 (1994)
Rocheford WE, Rehg T, Chau PC, Biotechnol. Lett., 8, 115 (1986)
Sanroman A, Chamy R, Nunez MJ, Lema JM, Enzyme Microb. Technol., 16(1), 72 (1994)
Silva SS, Santos JC, Carvalho W, Aracava KK, Vitolo M, Process Biochem., 38, 903 (2003)
Santos JC, Converti A, Carvalho W, Mussatto SI, Silva SS, Process Biochem., 40, 113 (2005)
Santos JC, Mussatto SI, Dragone G, Converti A, Silva SS, Biochem. Eng. J., 23, 1 (2005)
Palmqvist E, Hahn-Hagerdal B, Bioresour. Technol., 74, 25 (2000)
Carvalho W, Silvo SS, Santos JC, Converti A, Enzyme Microb. Technol., 32(5), 553 (2003)
Carvalho W, Silva SS, Converti A, Vitolo M, Biotechnol. Bioeng., 79(2), 165 (2002)
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Tranoudis I, Efron N, Contact Lens and Anterior Eye, 27, 177 (2004)
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Campbell I, Duffus JH, in Yeast - a practical approach, IRL Press, Oxford, Washington DC (1988)
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Song KH, Lee KR, Korean J. Chem. Eng., 24(1), 116 (2007)
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Yahashi Y, Horitsu H, Kawai K, Suzuki T, Takamizawa K, J. Ferment. Bioeng., 81(2), 148 (1996)
Carvalho W, Silva SS, Vitolo M, Mancilha IM, Z. Naturforsch., 55C, 213 (2000)
Carvalho W, Silva SS, Vitolo M, Felipe MGA, Mancilha IM, Z. Naturforsch, 57C, 109 (2002)
Carvalho W, Silva SS, Converti A, Vitolo M, Felipe MGA, Roberto IC, Silva MB, Mancilha IM, Appl. Biochem. Biotechnol., 98-100, 489 (2002)
