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Received December 28, 2007
Accepted February 27, 2008
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Fabrication of functional biomolecular layer using recombinant technique for the bioelectronic device
1Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea 2Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea 3Department of BioNano technology, Kyungwon University, Seongnam 461-701, Korea
jwchoi@sogang.ac.kr
Korean Journal of Chemical Engineering, September 2008, 25(5), 1115-1119(5), 10.1007/s11814-008-0182-3
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Abstract
A novel immobilization method of blue copper protein azurin on a gold surface was developed without a chemical linker using recombinant technique. Azurin was recombined with a cysteine anchors by site-directed mutagenesis (SDM) and used to effect the mutations, changing the codon for Leu39Cys (L39C) from CTG to TGC. The immobilization of the functionalized protein is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 76.3%, as compared to that of wild type azurin. The electrochemical property of the fabricated thin film was investigated by cyclic voltammetry (CV). As a result, cysteine-modified azurin can be used for making high quality protein film, and applied to the fabrication of nano-scale bioelectronics.
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Porter MD, Bright TB, Allara DL, Chidsey CED, J. Am. Chem. Soc., 39, 11808 (2000)
Dubrovsky TB, Hou Z, Stroeve P, Abbott NL, Anal. Chem., 71, 327 (1999)
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Son YJ, Park KH, Lee SY, Oh SJ, Kim CK, Choi BT, Park YC, Seo JH, Biotechnol. Bioprocess Eng., 12, 556 (2007)
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Nichols JD, Xiang S, Schindelin H, Rajagopalan KV, Biochemistry, 46, 78 (2007)
Foley TL, Burkart MD, Curr. Opin. Chem. Biol., 11, 12 (2007)
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Cavalleri O, Natale C, Stroppolo ME, Relini A, Cosulich E, Thea S, Novie M, Gliozzia A, Phys. Chem. Chem. Phys., 2, 4630 (2000)
Patel N, Davies MC, Hartshorne M, Heaton RJ, Roberts CJ, Tendler SJ, Williams PM, Langmuir, 13(24), 6485 (1997)
Jordan CE, Corn RM, Anal. Chem., 69, 1449 (1997)
Iwatani S, Iwane AH, Higuchi H, Ishii Y, Yanagida T, Biochemistry, 38, 10318 (1999)
Choi JW, Kim JS, Lee BH, Jang YH, Kim YJ, Mol. Cryst. Liq. Cryst., 463, 281 (2007)
Bott AW, Current Separations, 18, 2 (1999)
