Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received December 31, 2007
Accepted February 27, 2008
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Determination of meaty peptide in enzymatic hydrolyzate of beef protein by HPLC-MS
1College of Food Engineering & Biotechnology, Tianjin Key Laboratory of Food Nutrition & Safety, Tianjin University of Science & Technology, Tianjin 300457, China 2Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, China 3Tianjin Chunfa Food Ingredient Limited Company, Tianjin, 300300, China
Korean Journal of Chemical Engineering, September 2008, 25(5), 1120-1124(5), 10.1007/s11814-008-0183-2
Download PDF
Abstract
The purpose of this study is to detect beefy meaty peptide (BMP) in beef hydrolyzate. The synthesized BMP is used as a standard sample in the study. High performance liquid chromatography (HPLC)/ion trap electrospray ionization mass spectrometry (ESI-MS) with UV detection was applied in qualitative analysis of the peptides. Six beef protein enzymatic hydrolyzate samples were separated on a Surveyor HPLC system through a SUPELCO Discovery® C18 analytical column (5 μm, 15 cm×2.1 mm i.d.). The column was eluted at a flow rate of 0.2 mL/min in a linear gradient elution mode of acetronitrile-water solution with 0.1% trifluoroacetic acid. The concentration of acetronitrile was increased from 5% to 50% in 40 minutes. A Finnigan LCQ Advantage MAX instrument was used as detector to analyze with ESI-MS and ESI-MS/MS in positive mode. Among the six samples of beef protein enzymatic hydrolysate, the BMP is detected and confirmed in sample No.4 with a higher intensity of characteristic peak and is further investigated by ESI-MS/MS. As a result, BMP exists in sample No.4. The study proves that HPLC-ESI-MS/MS is a simple, rapid, sensitive method to analyze target peptides from complex polypeptides.
References
Yamasaki Y, Maekawa K, Agric. Biol. Chem., 42, 1761 (1978)
Wang K, Maga JA, Bechtel PJ, J. Food Sci., 61, 837 (1996)
Wang K, Maga JA, Bechtel PJ, Lebensm.-Wiss. u.-Technol, 28, 539 (1995)
Gomez-Ruiz JA, Ramos M, Recio I, J. Chromatogr. A, 1054, 269 (2004)
Lee YW, Jin YZ, Row KH, Korean J. Chem. Eng., 23(2), 279 (2006)
Cui LJ, Nithipatikom K, Campbell WB, Anal. Biochem., 369, 27 (2007)
Gorman JJ, Wallis TP, Pitt JJ, Mass Spectrum. Rev., 21, 183 (2002)
Yamashita K, Okuyama M, Watanabe Y, Honmab S, Kobayashia S, Numazawaa M, Steroids, 72, 829 (2007)
Tsimogiannis D, Samiotaki M, Panayotou G, Oreopoulou V, Molecules, 12, 593 (2007)
Favretto D, Frison G, Maietti S, Ferrara SD, Int. J. Legal Med., 121, 259 (2007)
Wang K, Maga JA, Bechtel PJ, J. Food Sci., 61, 837 (1996)
Wang K, Maga JA, Bechtel PJ, Lebensm.-Wiss. u.-Technol, 28, 539 (1995)
Gomez-Ruiz JA, Ramos M, Recio I, J. Chromatogr. A, 1054, 269 (2004)
Lee YW, Jin YZ, Row KH, Korean J. Chem. Eng., 23(2), 279 (2006)
Cui LJ, Nithipatikom K, Campbell WB, Anal. Biochem., 369, 27 (2007)
Gorman JJ, Wallis TP, Pitt JJ, Mass Spectrum. Rev., 21, 183 (2002)
Yamashita K, Okuyama M, Watanabe Y, Honmab S, Kobayashia S, Numazawaa M, Steroids, 72, 829 (2007)
Tsimogiannis D, Samiotaki M, Panayotou G, Oreopoulou V, Molecules, 12, 593 (2007)
Favretto D, Frison G, Maietti S, Ferrara SD, Int. J. Legal Med., 121, 259 (2007)
