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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received January 3, 2008
Accepted February 27, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis and comparison of cloning methods for the preparation of repetitive polypeptides

Hun Su Chu Cha Yong Choi Jong In Won^1†

School of Chemical and Biological Engineering, Seoul National University, Gwanak-gu, Seoul 151-742, Korea ¹Department of Chemical Engineering, Hongik University, Mapo-gu, Seoul 121-791, Korea

jiwon@hongik.ac.kr

Korean Journal of Chemical Engineering, September 2008, 25(5), 1125-1130(6), 10.1007/s11814-008-0184-1

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Abstract

Repetitive polypeptides, defined as a protein consisting primarily of tandemly repeated blocks of amino acid sequence, are widely used biomaterials. These repetitive polypeptides can be used in diverse biological fields including tissue engineering scaffolds, drug delivery systems, biomaterials, and DNA separation systems. The physical/chemical properties of the repetitive polypeptides can be improved by changing the composition of the repeated amino acid sequence. In this study, we introduced genetic methods for the production of repetitive polypeptides. By using recursive directional ligation (RDL) and controlled cloning method (CCM), multimerized genes were cloned and identified. Also, we compared the characteristics of recursive directional ligation (RDL) with those of controlled cloning method (CCM).

Keywords

Repetitive Polypeptide ELP Recursive Directional Ligation (RDL) Controlled Cloning Method (CCM)

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