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Received December 20, 2007
Accepted April 7, 2008
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Fermentation strategy for the production of poly(3-hydroxyhexanoate) by Aeromonas sp. KC014
1Department of Chemical Engineering and Materials Science, Yuan Ze University, Taiwan 2Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taiwan
Korean Journal of Chemical Engineering, November 2008, 25(6), 1422-1426(5), 10.1007/s11814-008-0233-9
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Abstract
Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)) was produced by Aeromonas sp. KC014 strain isolated from Taiwan in soil environment in the flask and fermentor cultures. The medium optimization, such as carbon source and nitrogen source, carbon-nitrogen ratio was conducted to obtain the optimum 3-hydroxyhexanoate content. The defined medium with dodecanoic acid as the carbon source and (NH4)2SO4 as the nitrogen source was obtained as the main medium. When cells grown in medium containing 30 g/L dodecanoic acid, 15 g/L sodium gluconate and 1 g/L soytone (C/N was 30/1) as final PHA concentration, the cell dry weight and HB content of 5.16 g/L, 14.0 g/L and 36.0%, respectively, were obtained. The maximum HHx/PHA content increased from 0.1% to 1.3% nearly 12-fold when the dissolved oxygen was decreased from 40% to 20%. P(3HB-co-3HHx) biosynthesis was triggered_x000D_
by the addition of limited nitrogen, phosphorus and magnesium to get a maximum HHx/PHA content of 14% in 95 hours.
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