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Received December 29, 2007
Accepted March 25, 2008
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Fluorometric analysis on physicochemical properties of phosphatidylcholine-based W/O microemulsion involved with enzymatic reactivity in phospholipid hydrolysis
Advanced Technology Research Center, Gunma University, 1-5-1 Tenjin-cho, Kiryu-city, Gunma-pref., 376-8515, Japan 1Course in Bioresource Utilization Sciences, Graduate School of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-8510, Japan, Korea
Korean Journal of Chemical Engineering, November 2008, 25(6), 1439-1443(5), 10.1007/s11814-008-0236-6
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Abstract
Abstract.Interfacial tension and fluorometric analysis were employed for the investigation of the interfacial local fluidity and the hydrophobicity of the micro water pool in the PC-based water-in-oil (W/O) microemulsion. These microenvironment properties strongly influenced the phospholipase A2 reactivity for phospholipid hydrolysis in the W/O microemulsion. The organic phase was prepared by mixing of isooctane as a main solvent and 1-butanol as a co-solvent._x000D_
The critical micelle concentration (CMC) was dramatically decayed from 9mM to 0.025mM by the increasing of the 1-butanol content. The local interfacial fluidity of the micro water pool was measured by using fluorescence polarity indicated by 1,6-Diphenyl-1,3,5-hexatriene-4'-trimethylammonium tosylate (TMA-DPH) and Coumarin 343 (C343). It was apparently increased with increasing the molar ratio of additive 1-butanol. In contrast, the hydrophobicity of the water pool measured by C343 was almost constant throughout the molar ratio of additive 1-butanol. Additive alcohol influenced the micro fluidity and enhanced reactivity of phospholipase A2 in lipid hydrolysis.
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Abuin E, Lissi E, Ceron A, Rubio MA, J. Colloid Interface Sci., 258(2), 363 (2003)
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Bhattacharyya K, Hara K, Kometani N, Uozu Y, Kajimoto O, Chem. Phys. Lett., 361(1-2), 136 (2002)
Yamazaki K, Imai M, Suzuki I, Biochem. Eng. J., 19, 171 (2004)
Yao C, Tang S, Zhang J, Yu Y, J. Mol. Catal. B: Enzymatic, 18, 279 (2002)
Talukder MMR, Hayashi Y, Takeyama T, Zamam M, Wu JC, Kawanishi T, Shimizu N, J. Mol. Catal. B: Enzymatic, 22, 203 (2003)
Han D, Rhee JS, Biotechnol. Bioeng., 28, 1250 (1986)
Naoe K, Tamai M, Kawagoe M, Imai M, Shimizu M, Trans IChemE, 75, 143 (1997)
Chattopadhyay A, Chemistry Physics Lipids, 122, 3 (2003)
Chaudhuri R, Sengupta PK, Mukherjee KKR, J. Photochem. Photobiol. A: Chemistry, 108, 261 (1997)
Yamasaki T, Kajimoto O, Hara K, J. Photochem. Photobiol. A: Chem., 156, 145 (2003)
Hakoda M, Shiragami N, Enomoto A, Nakamura K, Bioprocess Biosyst Eng., 25, 243 (2003)
Sugiura S, Ichikawa S, Sano Y, Nakajima M, Liu XQ, Seki M, Furusaki S, J. Colloid Interface Sci., 240(2), 566 (2001)
Bermejo R, Talavera EM, delValle C, Alvarez-Pez JM, Colloids Surfaces B: Biointerfaces, 18, 51 (2000)
Hasegawa R, Sugimura T, Suzaki Y, Shindo Y, Kitahara A, J. Phys. Chem., 98(8), 2120 (1994)
Hasegawa M, Sugimura T, Suzaki Y, Shindo Y, Colloids Surfaces A: Physicochem. Eng. Aspects, 109, 305 (1996)
Laane C, Boeren S, Vos K, Veeger C, Biotechnol. Bioeng., 30, 81 (1987)
Garti N, Lichtenberg D, Silberstein T, Colloids Surfaces A: Physicochem. Eng. Aspects, 128, 17 (1997)
Yamazaki K, Imai M, Suzuki I, Colloids Surfaces A: Physicochem. Eng. Aspects, 293, 241 (2007)
Harkins WD, Brown FE, J. Am. Chem. Soc., 38, 288 (1916)
idem., ibid., 41, 499 (1919)
Abuin E, Lissi E, Ceron A, Rubio MA, J. Colloid Interface Sci., 258(2), 363 (2003)
Mora MP, Tourne-Peteilh C, Charveron M, Fabre B, Milon A, Muller I, Chem. Phys. Lipids, 101, 255 (1999)
Silva L, Coutinho A, Fedorov A, Prieto M, Biochimica et Biophysica Acta - Biomembranes, 1758, 452 (2006)
Bhattacharyya K, Hara K, Kometani N, Uozu Y, Kajimoto O, Chem. Phys. Lett., 361(1-2), 136 (2002)
