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Received April 1, 2008
Accepted May 30, 2008
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Inhibition of melanogenesis by Erigeron canadensis via down-regulating melanogenic enzymes in B16F10 melanoma cells
Department of Biological Engineering, Inha University, Incheon 402-751, Korea
ekkim@inha.ac.kr
Korean Journal of Chemical Engineering, November 2008, 25(6), 1463-1466(4), 10.1007/s11814-008-0240-x
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Abstract
The effects of Erigeron canadensis extract on melanogenesis and cell toxicity in cultured B16F10 mouse melanoma cells were investigated. E. canadensis extract down regulated melanin synthesis effectively at a non-toxic concentration. Its extract was fractionated by using a recycling HPLC with GS310 column (21.5×500 mm, 10-15 μM) into five fractions. The fraction 1 showed melanin inhibition by 48.0% at 100 mg/ml which was 2.5 times more efficient than the depigmenting effect of commercial arbutin (17.5%) and also did not show cell toxicity. To elucidate the depigmenting mechanism of fraction 1, in vitro and cellular tyrosinase activity, antioxidant activity, and protein level of the main melanogenic enzymes, such as tyrosinase, TRP-1 and TRP-2 were evaluated. Fraction 1 inhibited melanin synthesis in B16F10 melanoma cells by decreasing protein levels of melanogenic enzymes, especially tyrosinase. In_x000D_
conclusion, we suggest that this fraction may be a safe and effective depigmentation agent.
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Khan SB, Haq AU, Afza N, Malik A, Khan MTH, Shah MR, Choudhary MI, Chem. pharm. Bull., 53, 86 (2005)
Masamoto Y, Murata Y, Baba K, Shimoishi Y, Tada M, Takahata K, Biol. Pharm. Bull., 27, 422 (2004)
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