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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received April 9, 2008
Accepted May 30, 2008

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Simple fabrication of functionalized surface with polyethylene glycol microstructure and glycidyl methacrylate moiety for the selective immobilization of proteins and cells

Hwan-Moon Song Chang-Soo Lee^†

Department of Chemical Engineering, Chungnam National University, 220 Gung-dong, Yu-Seong gu, Daejeon 305-764, Korea

rhadum@cnu.ac.kr

Korean Journal of Chemical Engineering, November 2008, 25(6), 1467-1472(6), 10.1007/s11814-008-0241-9

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Abstract

This study reported simple surface modification for the immobilization of biomolecules such as proteins and cells onto desired area at micron-scale level. First, thin film composed of glycidyl methacrylate (GMA) was prepared by UV-photopolymerization. Then, the polyethylene glycol (PEG) microstructures which played a role in the prevention of nonspecific binding of biomolecules were fabricated by using micromolding in capillaries (MIMIC). Thus, we could easily obtain an orthogonal surface having biomolecular attraction and repulsion areas. In addition, we could control of the height of prepared PEG microstructures with spin coating or not. For the investigation of feasibility of biomolecule patterning onto the functionalized surface, FITC-BSA and HEK 293 were examined as representative biomolecule models. A functionalized surface with GMA promotes the strong adhesion of biomolecules, and PEG microstructures located on the background prevent nonspecific binding of biomolecules at micron-scale level._x000D_ The orthogonal difference in surface functionality showed strong possibility of simple patterning of biomolecules. In addition, the proposed method could easily control the size, shape, and height of patterns. It will be useful platform technology for the construction of a biomolecule array.

Keywords

Surface Modification Protein Patterning Cell Patterning Micromolding in Capillaries PEG

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