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Received April 22, 2008
Accepted June 26, 2008
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Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate
Department of Chemical and Biomolecular Engineering, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749, Korea
Korean Journal of Chemical Engineering, January 2009, 26(1), 127-130(4), 10.1007/s11814-009-0019-8
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Abstract
The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.
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